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Cloning and Expression Analysis of PePIP2 Gene from Passiflora edulia Sims
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Maohong AO1, Funing MA2, 3, Yang PENG1, Dongmei HUANG2, Wenting XING2, Bin WU2, Yi XU2, 3, *
Chinese Journal of Tropical Crops | 2023, 44(1) : 17 - 25
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Chinese Journal of Tropical Crops | 2023, 44(1): 17-25
• Omics & Biotechnology •
Cloning and Expression Analysis of PePIP2 Gene from Passiflora edulia Sims
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Maohong AO1, Funing MA2, 3, Yang PENG1, Dongmei HUANG2, Wenting XING2, Bin WU2, Yi XU2, 3, *
Affiliations
  • 1. Guizhou Subtropical Crop Institute, Xingyi, Guizhou 562400, China
  • 2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement, Haikou, Hainan 571101, China
  • 3. Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-season Reproduction Regions/Yazhou Bay Seed Laboratory, Sanya, Hainan 572000, China
Published: 2023-01-25 doi: 10.3969/j.issn.1000-2561.2023.01.003
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Passion fruit (Passiflora edulia Sims), as an emerging tropical fruit tree, plays a very important role in tropical agriculture. However, abiotic stresses such as drought and high temperature seriously affect its normal growth and development. Relevant studies have shown that aquaporin (AQP) can improve the stress resistance of plants. In this study, Passiflora 'Tainong' was used as the material, an aquaporin gene PePIP2 was cloned using the genome data of passion fruit. The open reading frame (ORF) was 861 bp, encoding 286 amino acids. The molecular formula was C1417H2156N360O373S8, the molecular weight was 30 459.37 Da, the isoelectric point was 8.84, and the subcellular localization was on the cell membrane. Analysis of the promoter region revealed that it contained cis-acting elements involved in stress response. The expression analysis showed that PePIP2 could be induced by drought, high temperature and low temperature stress. Among them, the expression level was the highest when the soil water content was 50%, treated at 45℃ for 4 h and at 0℃ for 48 h. Transiently expressed in tobacco, the expression of PePIP2 was also induced to varying degrees under drought stress at different times. The results of this study would lay the foundation for the analysis of the stress resistance mechanism and molecular breeding of the passion fruit.

passion fruit (Passiflora edulia Sims)  /  aquaporin  /  cloning  /  bioinformatics  /  abiotic stress  /  express
Maohong AO, Funing MA, Yang PENG, Dongmei HUANG, Wenting XING, Bin WU, Yi XU. Cloning and Expression Analysis of PePIP2 Gene from Passiflora edulia Sims[J]. Chinese Journal of Tropical Crops, 2023 , 44 (1) : 17 -25 . DOI: 10.3969/j.issn.1000-2561.2023.01.003
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Year 2023 volume 44 Issue 1
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.01.003
  • Receive Date：2022-04-24
  • Online Date：2026-03-05
  • Published：2023-01-25
Article Data
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History
  • Received：2022-04-24
  • Revised：2022-05-23
Funding
Affiliations
    1. Guizhou Subtropical Crop Institute, Xingyi, Guizhou 562400, China
    2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement, Haikou, Hainan 571101, China
    3. Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-season Reproduction Regions/Yazhou Bay Seed Laboratory, Sanya, Hainan 572000, China

Corresponding:

*XU Yi，E-mail: 。
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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