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Heterologous Synthesis the Precursors of Anticancer Drug Cucurbitacins in Nicotiana benthamiana
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Zhaokuan GUO1, 2, 3, Yina WANG1, 2, 3, Zhiyuan LI1, 2, 3, Xiaolin FENG1, 2, 3, Geng CHEN1, 2, 3, Yanyu SHU1, 2, 3, Guanghui ZHANG2, 3, Bing HAO2, 3, Shengchao YANG2, 3, *
Chinese Journal of Tropical Crops | 2023, 44(1) : 198 - 207
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Chinese Journal of Tropical Crops | 2023, 44(1): 198-207
• Post-harvest Treatment & Quality Safety •
Heterologous Synthesis the Precursors of Anticancer Drug Cucurbitacins in Nicotiana benthamiana
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Zhaokuan GUO1, 2, 3, Yina WANG1, 2, 3, Zhiyuan LI1, 2, 3, Xiaolin FENG1, 2, 3, Geng CHEN1, 2, 3, Yanyu SHU1, 2, 3, Guanghui ZHANG2, 3, Bing HAO2, 3, Shengchao YANG2, 3, *
Affiliations
  • 1. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
  • 2. National and Local Joint Engineering Research Center for Germplasm Innovation and Utilization of Southwest Chinese Medicinal Materials, Yunnan Agricultural University, Kunming, Yunnan 650201, China
  • 3. Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan 650106, China
Published: 2023-01-25 doi: 10.3969/j.issn.1000-2561.2023.01.020
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The elucidation of cucurbitacin biosynthesis pathway is essential for heterologous production due to its significant anticancer activity. The biosynthesis of cucurbitacin starts from cucurbitadienol (Cuol) catalyzed by cucurbitadienol synthase (CBS) of the oxysqualene cyclase (OSCs) family to form 2,3-oxysqualene which subsequently catalyzed by cytochrome P450 (CYP) and other enzymes for oxidative modification to produce various cucurbitacins. In this study, an efficient transient expression system mediated by Agrobacterium tumefaciens was established. The CBS gene (HcOSC6) from Hemsleya chinensis and truncated 3-hydroxy-3-methyl-glutaryl coenzyme A reductase gene (tHMGR) from Avena strigose were continuously optimized in the tobacco co-expression system to increase Cuol yield from 2.832 mg/g (DW) to 9.48 mg/g (DW) in Nicotiana benthamiana leaves. Meanwhile, HcCYP87D20 of H. chinensis was also co-infiltrated into tobacco leaves to obtain 11-carbonyl-20β-hydroxy-cucurbitol. This study would provide a theoretical basis for efficient heterologous production of cucurbitacin.

cucurbitacin  /  transient expression  /  Hemsleya chinensis Cogn  /  Nicotiana benthamiana
Zhaokuan GUO, Yina WANG, Zhiyuan LI, Xiaolin FENG, Geng CHEN, Yanyu SHU, Guanghui ZHANG, Bing HAO, Shengchao YANG. Heterologous Synthesis the Precursors of Anticancer Drug Cucurbitacins in Nicotiana benthamiana[J]. Chinese Journal of Tropical Crops, 2023 , 44 (1) : 198 -207 . DOI: 10.3969/j.issn.1000-2561.2023.01.020
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Year 2023 volume 44 Issue 1
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.01.020
  • Receive Date：2022-04-02
  • Online Date：2026-03-05
  • Published：2023-01-25
Article Data
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History
  • Received：2022-04-02
  • Revised：2022-06-20
Funding
Affiliations
    1. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
    2. National and Local Joint Engineering Research Center for Germplasm Innovation and Utilization of Southwest Chinese Medicinal Materials, Yunnan Agricultural University, Kunming, Yunnan 650201, China
    3. Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan 650106, China

Corresponding:

*YANG Shengchao，E-mail: 。
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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