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Cassava is an important food crop in tropical regions. Cassava bacterial blight caused by Xanthomonas phoseoli pv. manihotis (Xpm) is an important disease of cassava. Excavating and identifying the genes of cassava resistance to Xpm and analyzing its disease resistance mechanism are beneficial to the development of cassava disease-resistant germplasm. Plant heat shock transcription factors (Hsfs) play an important role in the process of plants resisting biotic and abiotic stresses. In this study, the full-length heat shock protein transcription factor gene MeHsfB3a was cloned from cassava cultivar 'Huanan 8' ('SC8') by RT-PCR technology. Bioinformatics analysis found that MeHsfB3a contained two exons and one intron, with a full length of 729 bp, encoding 242 amino acids with 27.9 kDa and pI=7.59. The theoretical instability coefficient was 56.86, which is an unstable protein, and the average hydrophilicity index was-0.880, indicating that the protein has good water solubility, and the fat solubility index was 65.98. The protein was predicted to localize in the nucleus. qRT-PCR analysis found that MeHsfB3a was expressed in young leaves, mature leaves, terminal buds, petioles, tuberous roots and fibrous roots, with the highest expression in mature leaves and less in other organs. The expression of MeHsfB3a was analyzed after 0 h, 3 h, 6 h, 1 d, 3 d and 6 d after infection of cassava 'SC8' leaves by pathogen XpmHN11, and it was found that the expression of this gene was significantly increased after 1 d. This indicated that MeHsfB3a was involved in the response of 'SC8' cassava to XpmHN11. The MeHsfB3a gene of cassava 'SC8' was silenced by the VIGS technology, and the gene silencing efficiency reached 68.26%-82.44%. The leaves of silent plants were inoculated with XpmHN11, and the disease incidence was analyzed on 0 d, 3 d, and 6 d of inoculation, and it was found that the area of disease spots of the silent plants was significantly higher than that of the control. This study identified the heat shock protein transcription factor gene MeHsfB3a involved in the process of cassava resistance to XpmHN11, which would help to further analyze the disease resistance mechanism of cassava to cassava bacterial blight.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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