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Selection and Verification of the Reference Genes of Euphorbia maculata Based on Transcriptome Sequencing
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Jianfeng XU1, Mingming GUI2, Yongkang ZHANG1, Zizhao YAO1, Shenghe HUANG2, Zechang RAO1, *
Chinese Journal of Tropical Crops | 2023, 44(11) : 2273 - 2280
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Chinese Journal of Tropical Crops | 2023, 44(11): 2273-2280
• Omics & Biotechnology •
Selection and Verification of the Reference Genes of Euphorbia maculata Based on Transcriptome Sequencing
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Jianfeng XU1, Mingming GUI2, Yongkang ZHANG1, Zizhao YAO1, Shenghe HUANG2, Zechang RAO1, *
Affiliations
  • 1.Fuzhou Medical College, Nanchang University, Fuzhou, Jiangxi 344000, China
  • 2.Department of Basic Medicine, Jiangxi College of Traditional Chinese Medicine, Fuzhou, Jiangxi 344000, China
Published: 2023-11-25 doi: 10.3969/j.issn.1000-2561.2023.11.016
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The flavonoid quercetin is one of the main active ingredients in Chinese medicinal herb, Euphorbia maculata. At present, there are few reports on quercetin biosynthesis gene, and real-time quantitative PCR (RT-qPCR) is one of the common methods in gene research. But it is a prerequisite for relative quantitative RT-qPCR to have suitable reference genes. In order to obtain a suitable reference gene, this experiment was based on the results of transcriptome sequencing, EmSE, EmUBC, EmGDI1, EmLSM12, EmGBP, EmSYP22, EmTRI1, EmRSZ21, EmRPL7 and EmUBQ obtained by our research group in the early stage were selected as candidate reference genes. RT-qPCR was used to detect the expression of the candidate reference genes in roots, stems, leaves and fruits of E. maculata during vegetative and reproduction stages. The expression stability of the candidate genes was analyzed using geNorm, NormFinder and BestKeeper software. The results of different software were integrated and ranked by the geometric mean method. The ranking was in the order EmGDI1>EmUBQ>EmSE>EmUBC>EmLSM12=EmGBP>EmTRI1>EmSYP22>EmRSZ21=EmRPL7. EmC4H, a key enzyme gene in quercetin biosynthesis, was analyzed using EmGDI1 as the reference gene. The difference of EmC4H expression in different tissues at different stages was consistent with the trend of transcriptome sequencing. The reference gene EmGDI1 developed in this study could be used as a suitable reference gene for tissue-specific expression studies of different stages of E. maculata.

Euphorbia maculata  /  reference gene  /  transcriptome sequencing  /  RT-qPCR
Jianfeng XU, Mingming GUI, Yongkang ZHANG, Zizhao YAO, Shenghe HUANG, Zechang RAO. Selection and Verification of the Reference Genes of Euphorbia maculata Based on Transcriptome Sequencing[J]. Chinese Journal of Tropical Crops, 2023 , 44 (11) : 2273 -2280 . DOI: 10.3969/j.issn.1000-2561.2023.11.016
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Year 2023 volume 44 Issue 11
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.11.016
  • Receive Date：2022-09-19
  • Online Date：2026-03-05
  • Published：2023-11-25
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  • Received：2022-09-19
  • Revised：2022-11-17
Funding
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    1.Fuzhou Medical College, Nanchang University, Fuzhou, Jiangxi 344000, China
    2.Department of Basic Medicine, Jiangxi College of Traditional Chinese Medicine, Fuzhou, Jiangxi 344000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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