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Selection of Suitable RT-qPCR Reference Genes for Floral Scent Biosynthesis in Phalaenopsis I-Hsin Venus
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Yangting ZHANG1, Yanping ZHANG1, Jingyan HUANG1, Wenjun WANG1, Yan TONG1, Kai ZHAO2, Yuzhen ZHOU1, *
Chinese Journal of Tropical Crops | 2023, 44(11) : 2188 - 2195
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Chinese Journal of Tropical Crops | 2023, 44(11): 2188-2195
• Special Topic of Orchid Science •
Selection of Suitable RT-qPCR Reference Genes for Floral Scent Biosynthesis in Phalaenopsis I-Hsin Venus
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Yangting ZHANG1, Yanping ZHANG1, Jingyan HUANG1, Wenjun WANG1, Yan TONG1, Kai ZHAO2, Yuzhen ZHOU1, *
Affiliations
  • 1.College of Landscape Architecture and Art, Fujian Agricultural and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Protection and Utilization, Fuzhou, Fujian 350002, China
  • 2.College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China
Published: 2023-11-25 doi: 10.3969/j.issn.1000-2561.2023.11.006
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Phalaenopsis I-Hsin Venus is an excellent variety of fragrant flowers, with elegant flower types, long flowering period and rich floral fragrance, which has high garden ornamental value. ACT1, ACT2, ACT3, GAPDH, EF1α, TUA, TUB and Ubi, were selected as the candidate internal reference genes based on the transcriptome data of different flower development stages in Phal. I-Hsin Venus to select the appropriate reference genes for RT-qPCR analysis of the correlated genes in the biosynthesis pathway of the floral scent in Phalaenopsis I-Hsin Venus. The expression of the candidate internal reference genes in inflorescences at different stages was detected by RT-qPCR. The candidate internal parameter genes were analyzed by combining three internal parameter gene stability analysis software: geNorm, NormFinder and BestKeeper. The results of comprehensive analysis showed that ACT1 was the most stable and could be used as the best internal reference gene for the expression analysis of Phal. I-Hsin Venus.

Phalaenopsis I-Hsin Venus  /  reference genes  /  RT-qPCR  /  expression stability
Yangting ZHANG, Yanping ZHANG, Jingyan HUANG, Wenjun WANG, Yan TONG, Kai ZHAO, Yuzhen ZHOU. Selection of Suitable RT-qPCR Reference Genes for Floral Scent Biosynthesis in Phalaenopsis I-Hsin Venus[J]. Chinese Journal of Tropical Crops, 2023 , 44 (11) : 2188 -2195 . DOI: 10.3969/j.issn.1000-2561.2023.11.006
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doi: 10.3969/j.issn.1000-2561.2023.11.006
  • Receive Date：2022-06-05
  • Online Date：2026-03-05
  • Published：2023-11-25
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  • Received：2022-06-05
  • Revised：2022-09-15
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Affiliations
    1.College of Landscape Architecture and Art, Fujian Agricultural and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Protection and Utilization, Fuzhou, Fujian 350002, China
    2.College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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