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Establishment and Application of a Method for Genome Size Estimation of Piper L. Using Flow Cytometry
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Baoduo WU1, 3, 4, Lisong HU1, 3, 4, Jianxiong FENG2, Rui FAN1, 3, 4, Fen GUO2, Xunzhi JI1, 3, 4, Chaoyun HAO1, 3, 4, Lin YAN1, 4, *
Chinese Journal of Tropical Crops | 2023, 44(11) : 2265 - 2272
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Chinese Journal of Tropical Crops | 2023, 44(11): 2265-2272
• Omics & Biotechnology •
Establishment and Application of a Method for Genome Size Estimation of Piper L. Using Flow Cytometry
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Baoduo WU1, 3, 4, Lisong HU1, 3, 4, Jianxiong FENG2, Rui FAN1, 3, 4, Fen GUO2, Xunzhi JI1, 3, 4, Chaoyun HAO1, 3, 4, Lin YAN1, 4, *
Affiliations
  • 1.Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China
  • 2.College of Tropical Crop Science, Yunnan Agricultural University, Pu'er, Yunnan 665000, China
  • 3.Hainan Sim Soonliang Academician Workstation, Wanning, Hainan 571533, China
  • 4.Key Laboratory of Genetic Resources Utilization of Spiced Beverage Crops, Ministry of Agriculture and Rural Affairs, Wanning, Hainan 571533, China
Published: 2023-11-25 doi: 10.3969/j.issn.1000-2561.2023.11.015
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Six nuclear extracts and three leaves of different development stage were screened to establish the flow cytometry (FCM) method for determining the genomic size of Piper L. and Cucumis sativus L. was used as the DNA reference standard. Result showed that the modified nuclear extract and inverted leaf were suitable for the flow cytometry of Piper L. The genome size of cultivated varieties, including Kuching, Aman and EMAS, ranged from 667.94 to 719.32 Mb, and Reyin1, microphylla variety from Cambodia, Panniyur-1 and 73F5 ranged from 759.69 to 785.38 Mb. Therefore, the genome size was significantly different among cultivated varieties. Furthermore, it is speculated that the chromosome numbers of P. wallichii and P. hancei, with genome size larger than 900 Mb, should be larger than 52 with a multiple of 13, and the number of chromosomes of germplasm, with genome size ranged from 600 to 800 Mb, may be 52. The hybrid not only had similar phenotypes to cultivated varieties and P. flaviflorum, but also had an average genome size of cultivated varieties and P. flaviflorum. Therefore, it is speculated that the hybrid may be the hybrid offspring of cultivated varieties and P. flaviflorum. This study would provide a method for the determination of the genome size of Piper L. germplasm, and provide a technical basis for the study of Piper L. germplasm evaluation and distant hybridization.

Piper L.  /  flow cytometry  /  modified nuclear extract  /  genomic size
Baoduo WU, Lisong HU, Jianxiong FENG, Rui FAN, Fen GUO, Xunzhi JI, Chaoyun HAO, Lin YAN. Establishment and Application of a Method for Genome Size Estimation of Piper L. Using Flow Cytometry[J]. Chinese Journal of Tropical Crops, 2023 , 44 (11) : 2265 -2272 . DOI: 10.3969/j.issn.1000-2561.2023.11.015
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Year 2023 volume 44 Issue 11
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.11.015
  • Receive Date：2022-10-10
  • Online Date：2026-03-05
  • Published：2023-11-25
Article Data
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History
  • Received：2022-10-10
  • Revised：2022-11-16
Funding
Affiliations
    1.Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China
    2.College of Tropical Crop Science, Yunnan Agricultural University, Pu'er, Yunnan 665000, China
    3.Hainan Sim Soonliang Academician Workstation, Wanning, Hainan 571533, China
    4.Key Laboratory of Genetic Resources Utilization of Spiced Beverage Crops, Ministry of Agriculture and Rural Affairs, Wanning, Hainan 571533, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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