Molecular Cloning and Expression Pattern Analysis of S-adenosylmethionine Synthase Gene <i>CaSAMS3</i> in Coffee

Molecular Cloning and Expression Pattern Analysis of S-adenosylmethionine Synthase Gene CaSAMS3 in Coffee

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Xi'ao WANG¹^,²^,³^,⁴^,⁵, Zhenyang LIAO¹^,²^,³^,⁴^,⁵^,⁶, Na AN¹^,²^,³^,⁴^,⁵, Lisong HU¹^,²^,³^,⁴^,⁵^,⁶, Lifang HUANG¹^,²^,³^,⁴^,⁵, Xinchuan ZHAO⁷, Wenyan XIONG¹^,²^,³^,⁴^,⁵, Yongqing PANG¹^,²^,³^,⁴^,⁵, Yunlei WANG¹^,⁴^,⁵, Xiaoyang WANG¹^,²^,³^,⁴^,⁵, William Solano SANCHEZ³^,⁸, Lin YAN¹^,²^,³^,⁴^,⁵

Chinese Journal of Tropical Crops | 2026, 47(2) : 302 - 312

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Chinese Journal of Tropical Crops | 2026, 47(2): 302-312

• Omics & Biotechnology •

Molecular Cloning and Expression Pattern Analysis of S-adenosylmethionine Synthase Gene CaSAMS3 in Coffee

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Affiliations

^1.Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China

^2.Coffee Hainan Engineering Research Center, Wanning, Hainan 571533, China

^3.International Joint Research Center for Tropical Spice and Beverage, Wanning, Hainan 571533, China

^4.Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture & Rural Affairs, Wanning, Hainan 571533, China

^5.Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China

^6.National Key Laboratory of Tropical Crop Biological Breeding, Sanya, Hainan 572024, China

^7.College of Tropical Crops, Yunnan Agricultural University, Pu'er, Yunnan 665000, China

^8.Center for Tropical Agricultural Research and Higher Education, Turrialba 30501, Costa Rica

Published: 2026-02-25 doi: 10.3969/j.issn.1000-2561.2026.02.003

Outline

Abstract

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S-adenosylmethionine synthase (SAMS) is a key enzyme catalyzing the synthesis of S-adenosylmethionine (SAM, S-adenosylmethionine), widely involved in various biological processes in plants, such as epigenetic regulation, oxidative stress, and growth and development. In this study, the full-length cDNA sequence of CaSAMS3 was successfully cloned from coffee, and the physicochemical properties, protein structure, conserved domains, and evolutionary characteristics of the encoded CaSAMS3 protein were analyzed. Through cis-acting element analysis and protein interaction network prediction, and the metabolic processes involving CaSAMS3 were explored, and its expression pattern was analyzed in combination with qPCR and subcellular localization studies. The results showed that the open reading frame length of CaSAMS3 was 1173 bp, encoding a hydrophilic protein (molecular weight 42.57 kDa, theoretical isoelectric point 6.07), with a secondary structure mainly composed of α- helices and irregular curls, and located on cell membrane and nucleus. The protein contained a typical SAMS conserved domain and had the highest homology with SAMS in kiwifruit. Its promoter contained response elements related to light, hormones, stress, and growth and development, including MYB and MYC binding sites. CaSAMS3 exhibited tissue-specific expression, and its expression trend during developmental stages was consistent with accumulation pattern of caffeine. This study would provide an important theoretical basis for elucidating the biological function of CaSAMS3 in the regulation of caffeine synthesis, and provides a potential target for improving coffee quality and resistance.

Key words

coffee / caffeine / S-adenosylmethionine synthase / gene cloning / expression pattern

Cite this Article

Xi'ao WANG, Zhenyang LIAO, Na AN, Lisong HU, Lifang HUANG, Xinchuan ZHAO, Wenyan XIONG, Yongqing PANG, Yunlei WANG, Xiaoyang WANG, William Solano SANCHEZ, Lin YAN. Molecular Cloning and Expression Pattern Analysis of S-adenosylmethionine Synthase Gene CaSAMS3 in Coffee[J]. Chinese Journal of Tropical Crops, 2026 , 47 (2) : 302 -312 . DOI: 10.3969/j.issn.1000-2561.2026.02.003

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Year 2026 volume 47 Issue 2

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Article Info

doi: 10.3969/j.issn.1000-2561.2026.02.003

Receive Date：2025-11-03
Online Date：2026-06-26
Published：2026-02-25

Article Data

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History

Received：2025-11-03
Accepted：2025-11-18

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