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Cloning and Expression Analysis of CsSVP Genes in Cymbidium sinense
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Yibing WANG1, 2, Chuqiao LU1, Zengyu LIN1, Fengxi YANG1, *, Yaqin WANG2, *
Chinese Journal of Tropical Crops | 2025, 46(12) : 2855 - 2867
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Chinese Journal of Tropical Crops | 2025, 46(12): 2855-2867
Omics & Biotechnology
Cloning and Expression Analysis of CsSVP Genes in Cymbidium sinense
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Yibing WANG1, 2, Chuqiao LU1, Zengyu LIN1, Fengxi YANG1, *, Yaqin WANG2, *
Affiliations
  • 1.Institute of Environmental Horticulture, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory for Innovative and Comprehensive Utilization of Garden Flower Germplasm, Guangzhou, Guangdong 510640, China
  • 2.College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China
Published: 2025-12-25 doi: 10.3969/j.issn.1000-2561.2025.12.004
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As an important ornamental flower, Cymbidium sinense needs to undergo a long period of low temperature dormancy during its flower development to achieve flowering. SHORT VEGETATIVE PHASE (SVP) is a key regulator in plant flowering pathway and plays an important role in plant dormancy and flowering. In this study, three SVP homologous genes, named CsSVP1, CsSVP2 and CsSVP3, were cloned from Xiaoxiang. The results of bioinformatics analysis showed that the full-length of the three CsSVPs genes were 651 bp, 687 bp and 714 bp, encoding 216, 228 and 237 amino acids, respectively, and all of them contained conserved MADS-box and K-box domains. Amino acid sequence alignment and phylogenetic analysis showed that CsSVP1 had the highest similarity with CgSVP, CsSVP2 and CsSVP3 had the highest similarity with EpMADS18 and DhcSVP, respectively. CsSVP1 was closely related to CgSVP of C. goeringii. CsSVP2 was closely related to EpMADS18, while CsSVP3 was closely related to DhcSVP. Promoter cis-element analysis showed that the CsSVPs gene contained many light response, hormone response and other elements. The results of qRT-PCR showed that the three CsSVPs genes were expressed in roots, stems, leaves, flowers and fruits of C. sinense. The expression patterns of CsSVP1 and CsSVP3 genes were similar, which were accumulated in leaves and stems, and the expression levels in flowers and fruits were significantly decreased. The expression of CsSVP2 was the highest in fruit, followed by leaves, stems and flowers, suggesting that their biological functions may be quite different. At the same time, the expression in different floral organs was detected, and it was found that the three CsSVPs had the highest expression in sepals. Furthermore, the expression of CsSVP2 gene in different flower development stages was also detected. It was found that the expression level of CsSVP2 gene was the highest in the S1 stage of early development, and decreased with the development of flower organs, and the expression level was the lowest in the S5 stage of mature flowers. The overall expression levels of CsSVP1 and CsSVP3 in floral organs were not high. The expression level of CsSVP1 was the highest in S2 period and the lowest in S5 period. The expression level of CsSVP3 was the lowest in S2 period, and the expression level continued to increase in the late stage of flower development. In addition, low temperature treatment found that the expression of CsSVP1 and CsSVP2 increased slightly after low temperature treatment, while CsSVP3 decreased significantly. In ABA treatment, only the expression of CsSVP1 was significantly inhibited, while CsSVP2 and CsSVP3 did not respond. The results suggest that different CsSVPs genes have different functions and may play different roles in the process of low temperature dormancy promoting flowering of orchids, which would provide an experimental basis for further analysis of the specific functions of SVP genes in the process of flower development.

Cymbidium sinense  /  SVP  /  gene cloning  /  expression analysis
Yibing WANG, Chuqiao LU, Zengyu LIN, Fengxi YANG, Yaqin WANG. Cloning and Expression Analysis of CsSVP Genes in Cymbidium sinense[J]. Chinese Journal of Tropical Crops, 2025 , 46 (12) : 2855 -2867 . DOI: 10.3969/j.issn.1000-2561.2025.12.004
Year 2025 volume 46 Issue 12
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doi: 10.3969/j.issn.1000-2561.2025.12.004
  • Receive Date:2025-07-21
  • Online Date:2026-06-24
  • Published:2025-12-25
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History
  • Received:2025-07-21
  • Accepted:2025-08-28
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Affiliations
    1.Institute of Environmental Horticulture, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory for Innovative and Comprehensive Utilization of Garden Flower Germplasm, Guangzhou, Guangdong 510640, China
    2.College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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