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Identification and Target Gene Prediction of miRNA Responding to the Infection of Meloidogyne enterolobii in Local Resistant Xiaomila Pepper of Hainan, China
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Chang GAO1, Zhiyuan ZHANG2, Ziji LIU3, Meihong CHEN4, Jie ZHU1, *, Zhenmu CAO3, *
Chinese Journal of Tropical Crops | 2025, 46(7) : 1571 - 1583
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Chinese Journal of Tropical Crops | 2025, 46(7): 1571-1583
• Omics & Biotechnology •
Identification and Target Gene Prediction of miRNA Responding to the Infection of Meloidogyne enterolobii in Local Resistant Xiaomila Pepper of Hainan, China
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Chang GAO1, Zhiyuan ZHANG2, Ziji LIU3, Meihong CHEN4, Jie ZHU1, *, Zhenmu CAO3, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry (School of Agriculture and Rural Affairs / School of Rural Revitalization), Hainan University / Hainan Key Laboratory for Quality Regulation of Tropical Horticultural Crops, Haikou, Hainan 570228, China
  • 2.Hainan Research Institute, Zhejiang University, Sanya, Hainan 572025, China
  • 3.Institute of Tropical Crops Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 4.Hainan Binfen Horticulture Co., Ltd., Haikou, Hainan 571133, China
Published: 2025-07-25 doi: 10.3969/j.issn.1000-2561.2025.07.005
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Meloidogyne enterolobii is a pathogen causing significant damages to pepper production. Utilization of resistant cultivars is the most economical and environmentally friendly method to control this disease. A deeper understanding of the regulatory mechanisms of resistance to root-knot nematodes could facilitate the breeding process of disease-resistant cultivars. In previous studies, analysis of transcriptomic data of Hainan local M. enterolobii-resistant pepper germplasm CF25 before and after inoculation were carried out to explore metabolic pathways closely related to nematode resistance. In order to further explore the regulatory mechanisms of resistance, sRNA libraries of CF25 before and after inoculation with M. enterolobii were sequenced to identify a total of 133 differentially expressed miRNAs. Based on the criteria of P≤0.05 and |log2(FC)|≥3, 33 significantly differentially expressed miRNAs were identified, including 19 known miRNAs and 14 newly discovered miRNAs. Target gene prediction for the 33 miRNAs yielded a total of 373 target genes, with miR5658-z having the highest number of predicted target genes. GO and KEGG analyses showed that the target genes were mainly enriched in pathways related to plant-pathogen interactions and signal transduction. It is speculated that miRNA-mediated signal transduction and plant defense responses might be important reasons for the resistance of CF25 to M. enterolobii. To preliminarily verify the regulatory relationships between differentially expressed miRNA and the target genes, six differentially expressed miRNA and the target genes were selected for qRT-PCR validation. The results showed that the differential expression trends of miRNA and target genes were consistent with the high-throughput sequencing results and generally conformed to the negative regulation pattern between miRNA and the target genes. The results of this study suggest that miRNA may play significant roles in the defense response to root-knot nematodes and lay the foundation for furtherly elucidating the mechanisms of miRNA-mediated nematode resistance in pepper.

Capsicum frutescens  /  Meloidogyne enterolobii resistance  /  sRNA sequencing  /  miRNA  /  target gene
Chang GAO, Zhiyuan ZHANG, Ziji LIU, Meihong CHEN, Jie ZHU, Zhenmu CAO. Identification and Target Gene Prediction of miRNA Responding to the Infection of Meloidogyne enterolobii in Local Resistant Xiaomila Pepper of Hainan, China[J]. Chinese Journal of Tropical Crops, 2025 , 46 (7) : 1571 -1583 . DOI: 10.3969/j.issn.1000-2561.2025.07.005
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Year 2025 volume 46 Issue 7
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.07.005
  • Receive Date：2025-02-04
  • Online Date：2026-06-24
  • Published：2025-07-25
Article Data
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History
  • Received：2025-02-04
  • Accepted：2025-03-09
Funding
Affiliations
    1.School of Tropical Agriculture and Forestry (School of Agriculture and Rural Affairs / School of Rural Revitalization), Hainan University / Hainan Key Laboratory for Quality Regulation of Tropical Horticultural Crops, Haikou, Hainan 570228, China
    2.Hainan Research Institute, Zhejiang University, Sanya, Hainan 572025, China
    3.Institute of Tropical Crops Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    4.Hainan Binfen Horticulture Co., Ltd., Haikou, Hainan 571133, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2025.07.005
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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