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STAY-GREEN (SGR) is a key gene that regulates chlorophyll degradation in plants and plays an important role in controlling plant senescence. To investigate the regulatory role of the SGR gene in the fruit coloration process of pepper, the homologous gene cloning method was employed to amplify the CaSGR gene from pepper fruit cDNA using PCR technology in this study. Bioinformatics analysis was conducted on its protein sequence, and a virus-induced gene silencing (VIGS) vector was constructed to preliminarily verify the gene function. The results showed that ORF of CaSGR was 792 bp, encoding 263 amino acids. Bioinformatics analysis revealed that SGR was relatively conserved among Solanaceae crops, and SGR had a closer evolutionary relationship with SGR from tomato and potato. CaSGR VIGS vector was constructed and injected into pepper leaves, successfully obtaining silenced plants. The silenced plants exhibited leaf whitening, fruit color changed, and CaSGR expression levels reduced significantly. Additionally, the chlorophyll and β-carotene content in the fruits of the silenced plants increased markedly. This study would provide a foundation for further elucidating the molecular regulatory mechanisms of CaSGR in pepper fruit coloration and offer theoretical support for breeding new pepper varieties with high carotenoid content.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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