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Atg26 (or Ugt51) is a UDP-glucose: sterol glucosyltransferase, which regulates the biological process of sterol conversion to sterol glucoside (SG), participates in various cellular autophagy processes, and plays an important role in fungal growth, development and pathogenicity. In order to investigate the role of Atg26 in the growth, development and virulence of Peronophythora litchii, six Atg26 homologous proteins were identified through bioinformatics. The analysis of protein properties showed that the secondary structure of the protein was mainly random curling and had hydrophilic properties. The results of gene and protein structure analysis showed that except PlATG26a, the other ATG26 homologous genes of P. litchii had introns, which were mostly distributed at the end of the gene. All Atg26 homologous proteins contained the CAT catalytic domain, but did not have the PH (pleckstrin homology) and GRAM (glucosyltransferase, Rab-like GTPase activators, and myotubularins) domains that make up the PBD (phosphoinositide binding domain). Further phylogenetic and conserved motif analysis of Atg26 homologous proteins found that diversity existed among the Atg26 homologous proteins in P. litchii, but the CAT domain of Atg26 was conserved in different species. Protein interaction prediction and protein molecular docking analysis showed that the interacting proteins of PlAtg26 were mostly located in the cytoplasm or membrane, concentrated in the process of cell metabolism, and had catalytic activity, REDOX activity or binding ability, which also indicated that PlAtg26 may not require the binding ability of GRAM domain for localization. Quantitative real-time PCR (qRT-PCR) analysis showed that PlATG26b was up-regulated in zoospore stage and early infection, but had no significant difference in sporangium stage and late infection. This result also indicated that Atg26 played an important role in the asexual reproduction and infection of P. litchii. In conclusion, PlAtg26 and Atg26 homologues in fungi have significant structural differences and may show different biological functions from fungi.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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