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In order to investigate the biocontrol ability and mechanism of Burkholderia GXMZU-5 with quorum quenching activity, the biocontrol effect was verified through experiments on bacterial soft rot of banana fruits and potted seedlings (Dickeya zeae GR-1), and the biocontrol mechanism was verified by measuring the content of biofilm, the ability to remove extracellular polysaccharides, the ability to inhibit the metabolic activity of biofilm, and PCR amplification of QQ enzyme homologous genes. The results showed that GXMZU-5 could effectively degrade acylhomoserine lactone (AHLs) signal molecules and had AHLs quenching ability. In the biocontrol experiment on banana fruits, the surface of the mixed bacteria experimental group was healthier than that of the control group, with no obvious black spots or soft rot signs. The cross-section and longitudinal section results of banana fruits showed that the lesion area of the treatment group was significantly reduced. In the potted plant control experiment, there were no obvious signs of rot at the injection site of the mixed bacteria group, and the natural plant height recovered by 56% compared with the pathogen group, indicating that GXMZU-5 had a significant inhibitory effect on the disease caused by Dickeya zeae GR-1. The results for exploring of the biocontrol mechanism showed that the total content of biofilm and the content of extracellular polysaccharides in the mixed bacteria system were lower than those of the two bacteria cultured separately. The MTT method for measuring biofilm metabolism indicated that GXMZU-5 could effectively reduce the biofilm metabolic activity of Dickeya zeae GR-1 and occupied a dominant position in the mixed bacteria system. The exploration of the biocontrol mechanism indicated that GXMZU-5 could reduce the biomass and vitality of Dickeya zeae GR-1 biofilm through quenching effect, thereby obtaining biocontrol ability. The amplification of QQ enzyme homologous genes showed that GXMZU-5 had the aiiA lactonase gene and could quench AHLs signal molecules. This study verified the biocontrol ability of GXMZU-5 and preliminarily explored its biocontrol mechanism, providing a certain reference basis for the use of quenching bacteria to control plant bacterial diseases.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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