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Gene TFL1 belongs to the phosphatidylethanolamine binding protein (PEBP) family and is closely related to plant flowering regulation. To explore the functions of TFL1 gene in Dendrobium hybrid, based on the transcriptome data of floral in Dendrobium hybrid, a TFL1 gene was cloned and named DenTFL1. And the bioinformatic analysis protein structure and subcellular localization prediction, expression patterns analysis were done to explored the functional of DenTFL1. The results showed that the full length of DenTFL1 cDNA sequence was 522 bp, encoding 173 amino acids. Bioinformatics analysis showed that the molecular formula of DenTFL1 was C870H1354N246O249S6, the theoretical relative molecular mass of the protein was 19.43 kDa, and the theoretical isoelectric point was 7.82, and the protein was hydrophilic and structurally stable. Subcellular prediction showed that it was located in the cytoplasm, without signal peptide and transmembrane domain. The secondary structure was dominated by 61.85% of random curl, also contained 23.70% of the extended chain, 14.45% of α-helix. The three-dimensional structural model was basically consistent with the secondary structure. The conserved domain analysis showed that DenTFL1 had a conserved PEBP domain and amino acid sequence alignment and phylogenetic tree analysis showed that DenTFL1 was closely related to D. nobile and D. thyrsiflorum. The constructed protein network showed that DenTFL1 mainly interacted with AT5G63440, LFY, SOC1, AGL24, AGL8. Expression analysis showed that DenTFL1 was expressed in all tissues of different development stages of Dendrobium hybrid, and had the highest expression level in the stems of young and mature plantlet and the flower stalks, and had a lower expression level in the roots of young plantlet and the leaves and roots of medium plantlets. Moreover, it had a high expression in different development stage of inflorescence except 3.0 cm long inflorescence. It also showed high expression in 7.0 mm and 9.0 mm width floral bud and the tissues of fully open flower, but expressed lower in the tissues of early development stages of floral bud. The research could provide a reference for the flowering regulation of Dendrobium hybrid, and add relevant information for the study of TFL1.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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