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Applications and Prospects of Cryogenic Technology in the Conservation of Tropical Crop Germplasm Resources
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Minrui WANG1, 2, Yonglin JING1, 2, Langxin CHEN1, 2, Xiaobing WANG1, 2, Chunyang Meng1, 2, 3, Bilan HUANG1, 2, 3, Li XU1, 2, 3, **, Zhiying LI1, 2, 3, **
Chinese Journal of Tropical Crops | 2025, 46(3) : 611 - 628
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Chinese Journal of Tropical Crops | 2025, 46(3): 611-628
• Germplasm Resources, Genetics & Breeding •
Applications and Prospects of Cryogenic Technology in the Conservation of Tropical Crop Germplasm Resources
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Minrui WANG1, 2, Yonglin JING1, 2, Langxin CHEN1, 2, Xiaobing WANG1, 2, Chunyang Meng1, 2, 3, Bilan HUANG1, 2, 3, Li XU1, 2, 3, **, Zhiying LI1, 2, 3, **
Affiliations
  • 1.Tropical Crop Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 2.Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Key Laboratory of Biological and Cultivation of Herb Medicine (Haikou), Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
  • 3.National Gene Bank of Tropical Crops / Hainan in vitro Gene Bank of Tropical Crops / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, Danzhou, Hainan 571737, China
Published: 2025-03-25 doi: 10.3969/j.issn.1000-2561.2025.03.010
Outline
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Tropical region is rich in plant genetic diversity with great demand for the safe preservation of plant germplasm resources. Cryopreservation [with liquid nitrogen (LN)] is an important method for the long-term preservation of plant germplasm resources. Although cryopreservation protocol often requires optimization for different species, it ensures the use of minimum space and the regular LN supply to facilitate the long-term preservation of genetic resources once established, thereby greatly reducing the labor input and costs in the long run. The cryogenic technique has been studied for more than half a century and has achieved survival and regeneration of plant materials such as seeds, shoot tips and pollen, as well as cell suspension and callus tissue after cryopreservation. To fully introduce the use of cryopreservation to secure valuable tropical plant species and support the development of tropical agricultural sciences, this review first focuses on the shoot tip and dormant shoot segment cryopreservation to review the research history and technological development of plant cryopreservation. The research advances of cryopreservation in tropical plant species were emphasized using shoot tips, seeds (zygotic embryos), pollen, cell suspension, and callus. The importance of genetic stability analysis after cryopreservation was also introduced before highlighting the importance and prospects of applying cryopreservation for long-term preservation of shoot tips, recalcitrant seeds, and embryogenic tissues and cells of tropical crops.

tropical crops  /  cryopreservation  /  germplasm resources  /  genetic integrity
Minrui WANG, Yonglin JING, Langxin CHEN, Xiaobing WANG, Chunyang Meng, Bilan HUANG, Li XU, Zhiying LI. Applications and Prospects of Cryogenic Technology in the Conservation of Tropical Crop Germplasm Resources[J]. Chinese Journal of Tropical Crops, 2025 , 46 (3) : 611 -628 . DOI: 10.3969/j.issn.1000-2561.2025.03.010
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Year 2025 volume 46 Issue 3
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.03.010
  • Receive Date：2024-09-05
  • Online Date：2026-06-25
  • Published：2025-03-25
Article Data
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History
  • Received：2024-09-05
  • Accepted：2024-11-11
Funding
Affiliations
    1.Tropical Crop Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    2.Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Key Laboratory of Biological and Cultivation of Herb Medicine (Haikou), Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
    3.National Gene Bank of Tropical Crops / Hainan in vitro Gene Bank of Tropical Crops / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, Danzhou, Hainan 571737, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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