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Dickeya fangzhongdai is one of the main pathogens causing the devastating disease (soft rot disease) of Orchidaceae. Pathogenic genes play a crucial role in the infection process. Dickeya fangzhongdai Onc5 was isolated from the diseased plant (Oncidium, Gower Ramsey) in Fujian province. Pathogenic gene play an important role in the interaction between pathogens and plants. The purpose of this study was to larify the molecular biological characteristics of D. fangzhongdai Onc5 vfmE gene and analysis its role in the infection process, so as to provide theretical basis for further reveal the pathogenic mechanism of D. fangzhongdai Onc5 vfmE gene in the infection process. Using the D. fangzhongdai Onc5 DNA as template, of vfmE gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and its bioinformatics were analyzed. The expression of vfmE gene was analyzed during D. fangzhongdai Onc5 infecting Oncidium by quantitative reverse transcription PCR (RT-qPCR). The result showed that the ORF sequence of vfmE gene was 564 bp, encoding 187 amino acids with a molecular weight of 21.93 kDa and isoelectric point of 9.01 and a conserved AraC-superfamily domain. The vfmE protein was an unstable hydrophilic protein without signal peptide and transmembrane structure. The secondary structure of protein was mainly composed of α-helix (58.29%) and random coil (40.64%). Sequence similarity analysis revealed that the translated molecules showed high homology with other pathogens in the genus Dickeya (>86%). RT-qPCR analysis showed that vfmE gene was highly expressed continuously throughout the infection process of D. fangzhongdai Onc5, and the highest expression level was at 12 h. vfmE protein plays an important role in the infection of D. fangzhongdai Onc5. Research on the pathogeneisis-related gene vfmE in D. fangzhongdai Onc5 provide a theoretical basis for further research on the vfmE gene function and pathogenic regulation mechanism in Dickeya genus. Furthermore, such studies may be useful for finding new target to control plant soft rot disease.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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