Home Journals Articles Updates About
CN
image
收藏切换
Cloning and Subcellular Localization Analysis of HbbHLH48 Gene from Hevea brasiliensis Muell. Arg.
收藏切换
PDF
Xiaokang FAN1, 2, 3, Songle FAN1, Mingyang LIU1, Bingbing GUO1, Hong YANG1, Longjun DAI1, Yu ZHANG2, 3, *, Lifeng WANG1, *
Chinese Journal of Tropical Crops | 2025, 46(3) : 534 - 543
Less
收藏切换
Chinese Journal of Tropical Crops | 2025, 46(3): 534-543
• Omics & Biotechnology •
Cloning and Subcellular Localization Analysis of HbbHLH48 Gene from Hevea brasiliensis Muell. Arg.
Full
Xiaokang FAN1, 2, 3, Songle FAN1, Mingyang LIU1, Bingbing GUO1, Hong YANG1, Longjun DAI1, Yu ZHANG2, 3, *, Lifeng WANG1, *
Affiliations
  • 1.Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Labora-tory Incubation Base for Cultivation & Physiology of Tropical Crops / Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 2.Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572024
  • 3.College of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
Published: 2025-03-25 doi: 10.3969/j.issn.1000-2561.2025.03.003
Outline
收藏切换

Plant bHLH transcription factor is one of the key factor of ethylene and jasmonic acid hormone signal transduction pathway, and plays an important role in the yield formation and latex flow in rubber trees. A bHLH transcription factor was cloned from the leaves of Reyan73397 designed as HbbHLH48 (GenBank: PQ303617), with a total length of 1128 bp encoding 375 amino acids. Its encoded protein has the highest similarity to Arabidopsis AtbHLH48, and belongs to the XVII subgroup. Bioinformatics analysis showed that it contained the bHLH_SF superfamily domain, but did not contain the transmembrane domain and signal peptide. The expression levels in flowers, leaves and latex were higher than those in roots and stems. Mechanical injury treatment down-regulated its expression in tapping trees. Methyl jasmonate, salicylic acid, and ethephon treatments initially significantly upregulate their expression and then downregulate it, while the expression pattern with gibberellin treatment is opposite to them. Subcellular localization analysis revealed it to be in the nucleus. It can be seen that HbbHLH48 is a member of the plant bHLH transcription factor family, and its function in Hevea brasiliensis may be involved in the gibberellin process, thereby regulating the flowering process and latex biosynthesis process, laying the foundation for further in-depth research.

Hevea brasiliensis Muell. Arg.  /  HbbHLH48  /  clone  /  expression analysis  /  subcellular localization
Xiaokang FAN, Songle FAN, Mingyang LIU, Bingbing GUO, Hong YANG, Longjun DAI, Yu ZHANG, Lifeng WANG. Cloning and Subcellular Localization Analysis of HbbHLH48 Gene from Hevea brasiliensis Muell. Arg.[J]. Chinese Journal of Tropical Crops, 2025 , 46 (3) : 534 -543 . DOI: 10.3969/j.issn.1000-2561.2025.03.003
Appendix
Less
Year 2025 volume 46 Issue 3
PDF
111
56
Cite this Article
BibTeX
Article Info
doi: 10.3969/j.issn.1000-2561.2025.03.003
  • Receive Date：2024-11-08
  • Online Date：2026-06-25
  • Published：2025-03-25
Article Data
Affiliations
History
  • Received：2024-11-08
  • Accepted：2024-11-14
Funding
Affiliations
    1.Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Labora-tory Incubation Base for Cultivation & Physiology of Tropical Crops / Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    2.Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572024
    3.College of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
References
Share
https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2025.03.003
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House