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Coffee is one of three major beverage plants in the world, which is an important economic crop serving the revitalization and construction of rural areas in hot areas and national “the Belt and Road” initiative. Hainan coffee has good quality, high prices and good earnings. Currently, there is a phenomenon of using inferior products and impersonating others in the market, which is seriously damaged the brand image of Hainan coffee. Therefore, the development of Hainan coffee origin traceability identification technology is of great significance for promoting identification of Hainan coffee. In this study, we used inductively coupled plasma-mass spectrometry technology to determine the content of 13 mineral elements (K, Mg, Ca, Cu, Fe, Ba, Co, Mo, Ni, Zn, Se, Cd, As) in 68 coffee bean samples from main production areas of robusta coffee both domestically and internationally. Principal component analysis, orthogonal partial least squares-discriminant analysis and cluster analysis were used to classify the production areas of medium grain coffee beans in Hainan and abroad. A model for identifying the origin of medium grained coffee was established using linear discriminant analysis. The results showed that there was a significant correlation between different mineral element. And content of elements As, Ca, Fe, K, and Zn between Hainan and foreign production areas were significantly different; OPLS-DA analysis divided coffee samples into two groups, Hainan and foreign areas, based on composition characteristics of five elements. Fisher discriminant model established by using content of elements As, K, and Ca could distinguish robusta coffee beans from Hainan and abroad. The correct discriminant rates of cross test and back generation test were 92.3% and 96.2%, respectively. The accuracy of cluster analysis in identifying product origin was 89.7%. The above research results demonstrated that fingerprint characteristics of mineral elements could be used for origin tracing of Hainan coffee, which provided key technical support for brand protection of Hainan coffee in the future.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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