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Hongyang kiwifruit is a distinguished variety independently developed in China. It features a distinctive red coloration, possesses high sugar content and low acidity, and is favored for its rich aroma and unique flavor profile. The growth and development of fruits are influenced by a multitude of factors, among which sucrose non-fermentation-1-associated protein kinase 1 (SnRK1) plays a pivotal role in the regulation of carbohydrate metabolism and the response to both biotic and abiotic stresses. Therefore, in this study, we cloned the full-length cDNA of 2 genes in the SnRK1 family of Hongyang kiwifruit, named AcSnRK1.1 and AcSnRK1.2. The physical and chemical properties, gene structure, conserved motifs, protein conserved domains, and cis-acting elements of AcSnRK1.1 and AcSnRK1.2 were systematically analyzed using bioinformatics approaches. The expression characteristics of AcSnRK1.1 and AcSnRK1.2 genes in different tissues and fruits were quantitatively detected by real-time fluorescence. AcSnRK1.1 and AcSnRK1.2 genes contained an open reading frame of 1548 bp and 1545 bp, respectively, encoding proteins containing 515 and 514 amino acids with highly consistent sequences (96.31%) respectively. Both genes contained 10 exons and 2 introns. Bioinformatics analysis showed that AcSnRK1.1 and AcSnRK1.2 had high homology with other plant SnRK1 proteins. The amino acid sequence showed that STKc_AMPK_alpha, AMPK_C and UBA_SnRK1_plant were unique to the SnRK1 family. Evolutionary tree analysis showed that AcSnRK1.1 and AcSnRK1.2 belonged to the SnRK1 family. Tissue specific analysis showed that AcSnRK1.1 and AcSnRK1.2 were expressed in all tissues of Hongyang Kiwifruit, but the expression abundance was the highest in fruit. During fruit development, the expression levels of AcSnRK1.1 and AcSnRK1.2 decreased first and then increased. qRT-PCR analysis showed that the expression of AcSnRK1.1 and AcSnRK1.2 was responsive to ABA, GA3, ET and CPPU. With the increase of storage time, the expression levels of AcSnRK1.1 and AcSnRK1.2 showed a downward trend first and then an upward trend, suggesting that AcSnRK1 gene might regulate the expression of sucrose synthase. The results of this study indicate that AcSnRK1.1 and AcSnRK1.2 genes play an important role in the development and storage of Hongyang kiwifruit, which would lay a foundation for further elucidation of the functions of AcSnRK1.1 and AcSnRK1.2 genes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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