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Auricularia villosula Malysheva is a similar species of Auricularia heimuer F. Wu, B.K. Cui & Y. C. Dai, mainly distributed in high altitude areas of tropical and subtropical regions. It can adapt to the high temperature and high humidity climate in Guangxi. There are few reports on the domestication of A. villosula. In this paper, the fruiting bodies of auriculae collected in the field was used, and the molecular marker was established by sequence analysis. The effects of different temperature, carbon and nitrogen sources on the mycelia growth of A. villosula were explored through single factor tests, and the carbon and nitrogen sources of the medium were further optimized by an orthogonal test. The fruiting body of fungus was preliminarily identified as A. villosula (TY081). The results showed that the developed molecular labeling method could quickly and effectively identify A. villosula. The optimal temperature for mycelia growth of A. villosula TY081 was 30 ℃, the optimal carbon source was glucose, and the optimal nitrogen source was soybean powder. The optimized medium carbon and nitrogen combination was fructose, yeast powder, mycelium growth rate reached 0.92 mm/d. A. villosula TY081 was incubated at 28 ℃ in a culture bag mainly composed of sawdust, and the bag was full for 60 days. After puncturing, it was cultured in a greenhouse for 10 days to form the primordia. The fruity body growth temperature was 24 ℃, the humidity was 85%, and the yield of each bag was 20.943 g (dry weight).
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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