Plant peroxidase (POD) plays a role in plant development, hormone signaling, and stress response, but there are few research reports on the POD gene in mango. This study used mango genome data as a reference and employed bioinformatics methods to identify members of the POD gene family from multiple aspects, including protein characteristics, phylogenetic relationships, gene structure, promoter cis acting elements, and gene expression patterns. The expression patterns of POD gene family members under enhanced UV-B irradiation through transcriptome and quantitative real-time PCR (qPCR) experiments were analyzed. The mango POD (MiPOD) gene family had a total of 77 family members, and the genes were then mapped to 17 chromosomes and 2 scaffolds, encoding amino acids with a number of 206~500 aa, Stable proteins were accountted for the majority, most of them were hydrophilic proteins. Most MiPOD were predicted to localize in subcellular within chloroplasts, and classified into seven subgroups using phylogenetic analysis. The gene structure among members of the subgroup were similar. The results of collinearity analysis indicated that the proportion of MiPOD genes involved in segmental duplication was relatively high. It was speculated that it may be related to the expansion of the MiPOD family. The selection pressure analysis indicated that the K_a/K_s values of collinear genes were far less than 1, indicating that MiPODs may be mainly subjected to purifying selection during the evolutionary process. The MiPODs promoter region contained a large number of light responsive, hormone responsive, and stress responsive elements. MiPOD had different expression patterns, which may be related to its different protein functions. MiPOD exhibited different expression patterns during fruit growth and development under enhanced UV-B irradiation, with only MiPOD7 showing significant differences in expression levels among the highly expressed members. MiPOD7 expression levels were significantly higher than those of the control under UV-B stress, suggesting that it may play an important role in the response of mango fruit to UV-B stress. In summary, members of the MiPOD gene family may have evolved through segmental duplication and intron reduction patterns, and perform different functions by sensing different types of signals, thus forming different expression patterns. This research would lay the foundation for further studying the response mechanism of mango POD genes to different signals.