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Meloidogyne enterolobii is one of the most devastating pathogens in tomato (Solanum lycopersicum) production. Grafting with resistant rootstocks could help superior cultivars gain root-knot nematode resistance straightly. Previously, we identified a medium-resistant tomato rootstock cultivar Guizhen 1. In order to deepen the understanding to its resistance mechanism, we inoculated Guizhen 1 and susceptible cultivar Yala with M. enterolobii, and measured the proportion of root knots and nematode numbers in roots once every four days within 28 days. The root knot proportion of Yala reached 71.7%, which was kept under 36.0% in Guizhen 1. The root knot proportions between resistant and susceptible cultivars were significantly different on day 12, 16, 20, 24 and 28 after inoculation. Whereas the differences of nematode numbers between resistant and susceptible cultivars were not significant except on day 20. Thus it can be seen that the resistance of Guizhen 1 was not reflected by limiting the invasion of nematodes. The LC-MS based non-targeted metabolome of resistant and susceptible roots on 5th day after inoculation were measured. With the threshold of VIP>1 and P<0.05, 415 significantly different metabolites were gained, among which 280 were up-regulated and 135 were down-regulated. The top 8 classes of metabolites with largest number of metabolites were amino acid and its metabolites, heterocyclic compounds, benzene and its derivatives, organic acid and its derivatives, aldehyde, ketone and ester, fatty acyls, glycerol phospholipids, alcohol and amines. KEGG dataset were employed to do the metabolite pathway enrichment analysis. Glycerophospholipid metabolism, ascorbate and aldarate metabolism, nicotinate and nicotinamide metabolism, betalain biosynthesis and flavonoid biosynthesis pathways had relatively high level of enrichment, with the glycerophospholipid metabolism having the highest enrichment level. In glycerophospholipid metabolism pathway, there were several metabolites with significant differences between resistant and susceptible cultivars, which were phosphocholine, phosphatidylcholine, lysophosphatidylcholine, sn-glycero-3-phosphocholine, triethanolamine and diethanolamine. It was speculated that glycerophospholipid metabolism pathway might enhance the M. enterolobii resistance of Guizhen 1 at certain level. In this study, the histological and metabolomic fundamentals of M. enterolobii resistance were explored in tomato rootstock cultivar Guizhen 1, which would provide certain basis for the in-depth study on resistance mechanism in the future.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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