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The triose-phosphate/phosphate translocator (triose-phosphate/phosphate translocator, TPT) is a triose phosphate transporter that plays an important role in plant carbon metabolism pathways as well as abiotic stresses. Sucrose has a wide range of edible value and important economic value. Sugarcane is the main raw material for sugar production, and sucrose from sugarcane accounts for more than 80% of China's sugar production, so it is very important to achieve the goal of high yield and high resistance of sugarcane. In this study, the sugarcane variety ROC22 was used as the material to clone the sugarcane triose/phosphate transporter gene ShTPT, and the physicochemical properties, conserved domains, transmembrane structure prediction and protein sequence comparison of ShTPT were carried out by bioinformatics methods. The results showed that the CDS of ShTPT was 1221 bp, encoding 406 amino acids, the molecular weight of the protein was 43.63 kDa, and the isoelectric point (pI) was 9.80. It was rich in alanine and leucine, its instability coefficient was 42.51, and its hydrophilic coefficient was 0.583, which was an unstable hydrophobic protein. ShTPT did not contain signal peptides but had 9 transmembrane domains. Conserved domain prediction showed that ShTPT contained a tpt domain, which was consistent with the characteristics of the TPT family. The results of phylogenetic analysis showed that ShTPT was clustered with Sorghum bicolor SbTPT (XP_002454867.1) and Zea mays ZmTPT (NP_001105497.1) with homology of 97.04% and 94.13%, respectively. Further subcellular localization analysis of ShTPT showed that ShTPT was localized in chloroplasts. Tissue expression pattern analysis of sugarcane showed that ShTPT was mainly expressed in leaves, and the expression level in roots and stems was very low. Under the drought stress conditions simulated by PEG, the expression of ShTPT showed an upward trend, indicating that it responded to drought stress. The results of this study suggest that ShTPT is a chloroplast-localized transmembrane transporter, which may be involved in the transport of primary carbon metabolic compounds in sugarcane leaves and responsive to drought stress. This study preliminarily determined that ShTPT gene plays an important role in the transport of carbon assimilates in leaves and abiotic stress, which provides a theoretical basis for further study of its function.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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