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The level of cyanogenic glucosides content is an important trait for the edible quality of cassava, and Me-CYP79D2 is one of the key enzyme genes regulating cassava cyanogenic glucosides synthesis. This study used the edible variety SC9 as the material, cloned the MeCYP79D2 gene in the cytochrome P450 enzyme family of cassava, analyzed it using bioinformatics software, and detected its expression characteristics in different cassava germplasm tubers using real-time quantitative PCR. The cyanogenic glucosides content was measured using HPLC, and its correlation with the expression level of MeCYP79D2 gene was analyzed. The results showed that the length of the coding region sequence of MeCYP79D2 gene was 1625 bp, encoding 528 amino acids. It belonged to an unstable lipophilic hydrophilic protein with 38 phosphorylation sites. Sequence alignment indicated that the gene was highly conserved, and phylogenetic analysis showed that MeCYP79D2 was closely related to rubber and leprosy trees. There was a significant positive correlation between the expression of MeCYPD2 gene and the content of cyanogen glycosides in cassava roots of germplasms with different levels of cyanogen glycosides. After treatment with methyl jasmonate, the expression of MeCYP79D2 gene in roots was significantly upregulated and the content of cyanogenic glucosides was significantly increased. The results of this study indicates that MeCYP79D2 is involved in cyanogenic glucosides synthesis, providing a theoretical basis for studying the molecular mechanism of cassava cyanogenic glucosides biosynthesis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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