Home Journals Articles Updates About
CN
image
收藏切换
Cloning, Expression Analysis, and Functional Verification of the MePCS1 Gene in Cassava
收藏切换
PDF
Baojuan LIANG1, 2, 3, Yujian GE1, 2, 3, Jingyi HOU1, 2, Huimin ZHANG1, 2, 3, Zhixin REN1, 2, 3, Mengting GENG1, Yajie WANG2, 3, Jianchun GUO2, 3, *, Yuan YAO2, 3, *
Chinese Journal of Tropical Crops | 2024, 45(11) : 2243 - 2250
Less
收藏切换
Chinese Journal of Tropical Crops | 2024, 45(11): 2243-2250
• Omics & Biotechnology •
Cloning, Expression Analysis, and Functional Verification of the MePCS1 Gene in Cassava
Full
Baojuan LIANG1, 2, 3, Yujian GE1, 2, 3, Jingyi HOU1, 2, Huimin ZHANG1, 2, 3, Zhixin REN1, 2, 3, Mengting GENG1, Yajie WANG2, 3, Jianchun GUO2, 3, *, Yuan YAO2, 3, *
Affiliations
  • 1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
  • 3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences / National key Laboratory for Tropical Crop Breeding, Sanya, Hainan 572000, China
Published: 2024-11-25 doi: 10.3969/j.issn.1000-2561.2024.11.001
Outline
收藏切换

Phytochelatin synthase (PCS) is a key enzyme that catalyzes the synthesis of phytochelatin (PC). PC can reduce the toxicity of heavy metals to plants through complexation. Heavy metal pollution in soil increases the risk of cassava food safety. It is of great significance to study the reduction and control of heavy metals in cassava by analyzing the function of MePCS1 gene. In this study, the coding region of MePCS1 gene in cassava variety SC8 was cloned by PCR. The full length was 1512 bp, encoding 503 amino acids. Protein sequence analysis showed that MePCS1 protein was a hydrophilic protein, containing 41 phosphorylation sites and 2 glycosylation sites, without signal peptide. The phylogenetic tree showed that the cassava MePCS1 protein had the closest relationship with the PCS protein of the rubber tree. The qPCR analysis showed that the MePCS1 gene had the highest expression level in the stem. During the development of cassava roots, the expression level of MePCS1 gene was the highest in the root enlargement period. Lead (Pb) stress induced MePCS1 gene expression. MePCS1 gene can improve the tolerance of BY4741 yeast to Pb. The results of this study provide new information for further analysis of the functional characteristics of the MePCS1 gene and its mechanism for reducing Pb in cassava tuber roots.

phytochelatin synthases  /  heavy metal Pb stress  /  yeast expression
Baojuan LIANG, Yujian GE, Jingyi HOU, Huimin ZHANG, Zhixin REN, Mengting GENG, Yajie WANG, Jianchun GUO, Yuan YAO. Cloning, Expression Analysis, and Functional Verification of the MePCS1 Gene in Cassava[J]. Chinese Journal of Tropical Crops, 2024 , 45 (11) : 2243 -2250 . DOI: 10.3969/j.issn.1000-2561.2024.11.001
Appendix
Less
Year 2024 volume 45 Issue 11
PDF
77
37
Cite this Article
BibTeX
Article Info
doi: 10.3969/j.issn.1000-2561.2024.11.001
  • Receive Date：2024-04-24
  • Online Date：2026-06-26
  • Published：2024-11-25
Article Data
Affiliations
History
  • Received：2024-04-24
  • Revised：2024-05-09
Funding
Affiliations
    1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
    3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences / National key Laboratory for Tropical Crop Breeding, Sanya, Hainan 572000, China
References
Share
https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2024.11.001
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House