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Litchi (Litchi chinensis Sonn.) is one of the important economic crops in subtropical areas. However, it is difficult to store litchi after picking and easy to browning, which is one of the biggest problems in the preservation process. WRKY family is a common transcription factor in plants, which mainly regulates physiological processes such as plant stress response and maturation. In order to research the effect of WRKY on the ripening and senescence process of litchi fruit after harvest, this study took Feiziao litchi as the experimental object, and cloned LcWRKY47 gene. The gene length was 1077 bp. The protein contains WRKY conserved domain. The secondary structure is dominated by random curling, accounting for 65.64%, and the tertiary structure is dominated by random curling and α-helix. Through motif analysis and multiple sequence alignment, LcWRKY47 protein was found to be a class II WRKY transcription factor. Phylogenetic analysis showed that LcWRKY47 in litchi was closely related to DlWRKY47 in longan, which are all classified as Sapindaceae. Subcellular localization experiments showed that LcWRKY47 protein was localized in the nucleus. RT-qPCR analysis showed that after treatment with 2 mmol/L oxalic acid, the expression of LcWRKY47 gene in the peel and pulp of litchi showed the same trend, which increased first and then decreased, and the expression level of LcWRKY47 gene in the treatment group was significantly higher than that in the control group after storage, suggesting that this transcription factor plays a positive role in the regulation of oxalic acid in the ripening and senescence process of litchi fruit. Overexpression of LcWRKY47 gene showed that the expression level of LcWRKY47 in transgenic fruits was significantly higher than that in control fruits, and the senescence process and browning of transgenic litchi was significantly better than that in treatment group. In conclusion, LcWRKY47 transcription factor may play a regulatory role in the senescence and browning process of litchi.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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