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Thioredoxin (TRX), a redox regulatory protein, plays an important role in plant resistance to abiotic stress. In this study, HbTRXo2, a thioredoxin gene, was cloned from rubber tree (Hevea brasiliensis) using RT-PCR. HbTRXo2 contained a coding region of 594 bp encoding a protein of 197 amino acids. The predicted molecular weight and isoelectric point of HbTRXo2 was 21.90 kDa and 7.59, respectively. The conserved domain and phylogenetic analysis showed that HbTRXo2 had a conserved TRX domain and was clustered with other plant o-type thioredoxin, suggesting that HbTRXo2 belonged to o-type thioredoxin. Quantitative real-time PCR indicated that HbTRXo2 gene was expressed in root, bark, latex, mature leaf, senescent leaf, new shoot, female flower and male flower tissues of rubber tree, with significantly higher expression level in latex than in other tissues. Compared with the healthy rubber trees, the expression of HbTRXo2 in the bark and latex of tapping panel dryness trees was significantly reduced. Under cold, polyethylene glycol (PEG)-induced drought, and oxidative stress induced by hydrogen peroxide (H2O2) and methyl violet (MV), the expression of HbTRXo2 gene was significantly up-regulated, indicating the involvement of HbTRXo2 in response to abiotic stress in rubber tree. To explore the function of HbTRXo2 in stress resistance, its yeast expression vector was constructed and transferred into Saccharomyces cerevisiae INVSC1 to obtain the recombinant yeast INVSC1 (pYES2-HbTRXo2). The survival differences between the recombinant yeast INVSC1 (pYES2-HbTRXo2) and the control yeast INVSC1 (pYES2) transformed with pYES2 empty vector after H2O2, PEG, and low temperature stress treatments were compared, and the results showed that the survival rate of INVSc1 (pYES2-HbTRXo2) was significantly higher than that of INVSC1 (pYES2) after PEG and H2O2 treatments, while the survival rate of INVSc1 (pYES2-HbTRXo2) was significantly lower than that of INVSC1 (pYES2) after low temperature stress treatment, indicating that the recombinant yeast transformed with HbTRXo2 gene improved the resistance to drought and oxidative stresses, but decreased the resistance to low temperature stress. The results demonstrate that HbTRXo2 plays an important role in latex production and latex flow, as well as abiotic stress resistance in rubber tree. The study would provide important references for further elucidating the biological function of HbTRXo2 in rubber trees.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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