PDF
Full
Outline
The aim of the study was to explore the anti-inflammatory activity of Qi-Nan essential oil (QEO) based on component analysis combined with the cellular inflammation model. The volatile components of QEO were analyzed by GC-MS. The antioxidant activity of QEO was detected by the measurement of DPPH radical scavenging and total antioxidant capacity. Bioinformatics was used to predict the targets and signaling pathways of QEO related to inflammation. NO and ROS levels were detected by fluorescent probe method. ELISA was used to detect the levels of inflammatory factors TNF-α, IL-6 and IL-1β. Western blot was used to detect the expressions of P-P65, P65, IκB-α and P-IκB-α. The main components of Qinan essential oil were 2-phenethyl-4H-chromen-4-one (50.08%), (-)-caryophyllene oxide (8.45%), patchulane (6.46%), elemol (3.88%), 2-methyl-6-(2-propenyl)-phenol (2.78%). The DPPH radicals scavenging of QEO was 51.98% at a concentration of 1 mg/mL and the total antioxidant capacity increased with the increase of QEO in the concentration range of 1-10 mg/mL. There were 533 intersecting targets of QEO and inflammation, and KEGG enrichment analysis showed that the main signaling pathways involved were MAPK, NF-κB and PI3K-Akt pathways. QEO significantly reduced the production and expression levels of NO, ROS, TNF-α, IL-6 and IL-1β (P<0.001). Meanwhile, the results of western blot showed that QEO also inhibited the expression of P-P65 and P-IκB-α proteins (P<0.001). Therefore, QEO can achieve anti-inflammatory activity through reducing the production of ROS, thereby inhibiting the activation of NF-κB signaling pathway and further drastically decrease the release of pro-inflammatory cytokines. The results demonstrate that QEO has the potential to be developed as an inflammation inhibitor.
PDF
85
39
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
