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Mutation breeding is one of the most promising approaches for increasing genetic and phenotypic variability in a short period of time. In order to explore the feasibility of creating a mango mutant gene library through mutagenesis. The study used Keitt mango as test materials, irradiated branches with 60Co-γ ray and soaked seeds in EMS solution to create a mutagenesis population, and analyzed the Genetic diversity by SC-SSR molecular markers. The results indicated that the seemi-lethal dose of 60Co-γ ray radiation is 31.37 Gy, and the semi-lethal time of immersion in 0.2% EMS solution is 5.9 hours. The genetic diversity of 100 mutagenized materials and Keitt material was analyzed through 16 pairs of SC-SSR primers, and a total of 117 polymorphic sites were detected. The average values of sexual locus, primer percentage of polymorphism bands, polymorphism information content (PIC), number of observed alleles (Na), number of effective alleles (Ne), Nei's gene diversity index (H) and Shannon's information index (I) were 93.18%, 0.610, 1.923,1.583,0.334 and 0.493, respectively. The genetic similarity coefficient among test materials ranged from 0.373 to 0.984, with an average of 0.779. The study indicated that mutagenesis significantly enhanced genetic diversity in mango, among which EMS mutagenesis seed variation was greater, and the results lay a solid theoretical foundation for mango mutation breeding.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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