PDF
Full
Outline
Powdery mildew caused by Erysiphe quercicola is a significant disease in China’s natural rubber industry, posing a severe threat to latex production. With the continuous advancement of predictive and forecasting technologies, the combination of qPCR detection and spore capture techniques holds promise as an efficient method for predicting the prevalence of powdery mildew. In this study, we focused on the microscale extraction of DNA from rubber tree powdery mildew spores. We compared various pre-treatment methods, different types of grinding beads with varying materials, and different types of grinding machines to identify the most suitable spore sample grinding system. Furthermore, we compared the efficiency of the CTAB method with several commercial extraction kits. Ultimately, we established a feasible microscale DNA extraction method for spores: first, the powdery mildew spore water solution subjected to 4 ℃ultrasonication for 10 min, subsequently, 2 mm zirconia beads (0.2 g), glass beads (0.4-0.6 mm, 0.2 g), and 0.2 mm zirconia beads (0.2 g) added, tissue grinder 70 Hz at 4 ℃, grind 40 s, stop 10 s, 20 cycles, finally, DNA extracted using the CTAB method. This method effectively and stably extracts DNA from 101 spores/mL rubber tree powdery mildew spores, without being affected by complex field conditions, and demonstrates high sensitivity and specificity. It provides a reliable theoretical basis and an effective technical approach for the prediction and forecasting of rubber tree powdery mildew.
PDF
88
45
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
