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Neuroprotective Effect and Mechanism of Arecoline on H2O2-induced Damage in SH-SY5Y Cell
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Yuan SUN1, 2, 4, Danyang WANG2, Jing SUN2, Yajuan BAI1, 2, 3, Bei FAN2, 3, Hongbo SONG4, Jianbang JI1, 3, 5, *, Cong LU1, 2, 3, 5, *, Fengzhong WANG1, 2, 3, *
Chinese Journal of Tropical Crops | 2024, 45(6) : 1252 - 1261
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Chinese Journal of Tropical Crops | 2024, 45(6): 1252-1261
• Post-harvest Treatment & Quality Safety •
Neuroprotective Effect and Mechanism of Arecoline on H2O2-induced Damage in SH-SY5Y Cell
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Yuan SUN1, 2, 4, Danyang WANG2, Jing SUN2, Yajuan BAI1, 2, 3, Bei FAN2, 3, Hongbo SONG4, Jianbang JI1, 3, 5, *, Cong LU1, 2, 3, 5, *, Fengzhong WANG1, 2, 3, *
Affiliations
  • 1.National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, Hainan 572024, China
  • 2.Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • 3.Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya, Hainan 572025, China
  • 4.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • 5.Haikou Key Laboratory of Areca Processing Research, Haikou, Hainan 570100, China
Published: 2024-06-25 doi: 10.3969/j.issn.1000-2561.2024.06.017
Outline
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The aim of this study was to explore the protective effect of arecoline on the cell model of H2O2-induced oxidative stress damaged model in SH-SY5Y cells and elucidate its mechanism. Cell viability was detected by CCK-8 assay. Spectrophotometry was used to detect LDH release, MDA, SOD, and CAT contents. Flow cytometry was used to detect cell apoptosis and mitochondrial membrane potential. Western blot was used to detect the expressions of Nrf2, HO-1, Keap1, Bcl-2, Bax and Caspase-3. Arecoline could reduce cell apoptosis and significantly increase mitochondrial membrane potential. The level of SOD elucidateand CAT increased while the level of MDA decreased. Arecoline 140 μmol/L group could significantly up-regulate the protein expression of Nrf2, HO-1 and Bcl-2 (P<0.001, P<0.0001), and down-regulate the protein expression of Keap1, Bax and Caspase-3 (P<0.001, P<0.0001). Conclusions: arecoline can effectively improve H2O2-induced oxidative stress injury in SH-SY5Y cell by activating the Nrf2/HO-1 signaling pathway, enhancing the activities of antioxidant enzymes and regulating the oxidation reduction system of cells, as well as regulating the Bcl-2/Bax/Caspase-3 signaling pathway and inhibiting cell apoptosis.

arecoline  /  SH-SY5Y  /  oxidative stress  /  neuroprotection  /  mechanism of action
Yuan SUN, Danyang WANG, Jing SUN, Yajuan BAI, Bei FAN, Hongbo SONG, Jianbang JI, Cong LU, Fengzhong WANG. Neuroprotective Effect and Mechanism of Arecoline on H2O2-induced Damage in SH-SY5Y Cell[J]. Chinese Journal of Tropical Crops, 2024 , 45 (6) : 1252 -1261 . DOI: 10.3969/j.issn.1000-2561.2024.06.017
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Year 2024 volume 45 Issue 6
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.06.017
  • Receive Date：2023-06-09
  • Online Date：2026-06-24
  • Published：2024-06-25
Article Data
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History
  • Received：2023-06-09
  • Revised：2023-08-31
Funding
Affiliations
    1.National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, Hainan 572024, China
    2.Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    3.Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya, Hainan 572025, China
    4.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    5.Haikou Key Laboratory of Areca Processing Research, Haikou, Hainan 570100, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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