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Natural rubber is an important strategic material and industrial raw material in China, which mainly originates from rubber tree. The growth cycle of rubber tree is long, and the quality of rubber seedling directly affects the development of rubber industry. The somatic embryo plantlet of rubber tree shows good consistency in the previous studies, and has the advantages of rapid growth and high yield. Hainan Innovation Base for production of Natural Rubber New Planting Material is the base of the current large-scale production of rubber tree somatic embryo plantlets of Reyan 73397 and Reyan 917. In this paper, in order to clarify the large-scale production of the somatic embryo plantlets of rubber tree, the plant height, stem diameter, leaves number and taproot length of Reyan 73397 somatic embryo plantlets from 2018 to 2020 were studied, the differences in each index of the two varieties in 2020 were, compared, and the classification of different varieties in different years were done. The proportions of plant height, stem diameter, leaf number and taproot length of Reyan 73397 were similar in each region from 2018 to 2020. There were no significant differences in plant height, leaf number and taproot length in three years. There were significant differences in stem diameter between 2018 and 2019, and the mean value of the three years was above 2.00 mm, and the difference between the maximum and minimum value was only 0.09 mm. The plant height of Reyan 73397 and Reyan 917 was the same, and the mean value of plant height of both varieties was 8.0 cm. The plant height of the two varieties was mainly 5.0-10.0 cm, accounting for 66.13% and 66.91%, respectively. The stem diameter range of Reyan 73397 and Reyan 917 was 0.80-4.23 mm and 1.13-3.73 mm, respectively. The mean value of the two varieties was 2.11 mm and 2.18 mm, respectively. The stem diameter of the two varieties was highly concentrated between 1.50-3.00 mm, 85.94% and 83.82%, respectively. The leaf number of Reyan 73397 and Reyan 917 was ranged from 0 to 6 and 1 to 4, respectively. The number of leaves of the two varieties was mostly 1 to 3, accounting for 88.82% and 96.32%, respectively. The rate of 2 leaves in Reyan 917 was as high as 52.94%. The average taproot length of Reyan 73397 was 9.5 cm, while that of Reyan 917 was 9.1 cm. The taproot length of both varieties concentrated in the range of 8.0-12.0 cm, accounting for 74.12% and 79.41%, respectively. There were no significant differences in plant height, stem diameter and leaves number between the two varieties in 2020, while there was significant differences in taproot length (P<0.05). The proportion of first-grade plantlets and second-grade plantlets of different varieties in different years (2018—2020) was 19%-22% and 56%-62%. It is concluded that the mass production of rubber tree somatic embryo plantlet is stable, which could provide reference for the popularization of the production technology of rubber tree somatic embryo plantlet.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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