Fenjiaren is a new Dendrobium variety bred by Sanming Academy of Agricultural Sciences for both ornamental and edible purposes. In factory seedling cultivation, it was found that Fenjiaren had slow cluster bud proliferation, low proliferation coefficient, underdeveloped root system of tissue culture seedlings and other problems, which affecting the large-scale production of this variety. To improve the efficiency of factory seedling cultivation of the variety, the sterile seedlings were used as the explants, the effects of plant growth regulators including 6-BA, NAA, and additives such as banana puree and AC on the proliferation of stem segments and the rooting of seedlings were studied using L₉(3³) orthogonal experiments. The suitable culture medium for the proliferation of stem segments of Fenjiaren was 1/2 MS+6-BA 3 mg/L, banana puree 100 g/L, white sugar 30 g/L, activated carbon AC 1 g/L, and agar powder 5.6 g/L. After 4 months of cultivation, the proliferation coefficient of the stem segment was 5.08. Range analysis indicated that the primary and secondary relationship of the factors was 6-BA>NAA>banana puree. The inter subject effect test showed that 6-BA had a significant impact on the growth rate coefficient (P<0.05). The most suitable culture medium for rooting was 1/2 MS+NAA 1 mg/L+banana puree 100 g/L+white sugar 15 g/L+agar powder 5.6 g/L. After 2 months of cultivation, the growth rate coefficient of the rooting was 12.82, the average plant height of the seedling was 12.66 cm, the average leaf length was 4.02 cm, the average number of roots was 11.80, and the average plant weight was 1.67 g. Range analysis indicated that the primary and secondary relationship of the factors was NAA>AC>banana puree. This study optimized the proliferation and rooting conditions of a new variety of Fenjiaren, which could provide technical reference for the seedling breeding of new varieties of Dendrobium and other Orchid plants.

Dendrobium  /  Fenjiaren  /  tissue culture  /  proliferation  /  rooting