Phalaenopsis-hybrid Dendrobium is an important ornamental plant in tropical area and has high market values, with beautiful flower appearance and abundant flower colors. In this study, the protocorms and young seedling’s stem segments of commercial varieties, Den. Yaya Victoria, Den. Swirl and Den. Sonia Hiasakul, were used as the explants. The effects of different phytohormone combination on embryogenic callus induction, adventitious bud differentiation and plantlet regeneration were investigated. During the embryogenic callus (EC) induction stage, the best induction explants were protocorms and 2-month-old seedling stem segments and EC could be obtained after 30 days culture in dark condition. The best medium for EC induction of was MSD (MS+30 g/L glucose+1 g/L Hyponex+8 g/L agar) as the basic medium with 0.5 mg/L KT and 0.2-0.5 mg/L 2,4-D. The highest average induction rate of EC was 31.11%, 22.78%, and 50% for Den. Yaya Victoria, Den. Swirl and Den. Sonia Hiasakul, and the induction time for Den. Yaya Victoria was only 15 days from explants to EC. The best proliferation medium was MSD+0.5 mg/L IBA+0.5 mg/L KT, which maintaining EC in good condition without differentiation and the multiplication rate was 0.9. The best medium for differentiation was 1/2 MS+0.5 mg/L IBA+0.15 mg/L KT under light condition of 16 h one day, EC quickly differentiating and forming 2-3 leaf adventitious buds within 30 days, and finally regenerating into plantlet after 60 days in the same medium. The best rooting medium was 1/2 MS+30 g/L sugar+0.5 mg/L NAA+8 g/L agar, with rooting rate 100% and five roots in length 1.3 cm per plantlet. In this research, the EC induction, proliferation and differentiation media have a wide-spectrum and the EC regeneration pathway could lay a solid foundation for further molecular breeding of Phalaenopsis-hybrid Dendrobium.