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Separation and Purification of Ergothioneine from Natural Rubber Serum with Ion Exchange Resin
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Shikuan JIANG1, Juan LI2, Guimei ZHANG1, Rong XU1, Li DING3
Chinese Journal of Tropical Crops | 2024, 45(5) : 1000 - 1006
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Chinese Journal of Tropical Crops | 2024, 45(5): 1000-1006
• Post-harvest Treatment & Quality Safety •
Separation and Purification of Ergothioneine from Natural Rubber Serum with Ion Exchange Resin
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Shikuan JIANG1, Juan LI2, Guimei ZHANG1, Rong XU1, Li DING3
Affiliations
  • 1.Yunnan Institute of Tropical Crops / Yunnan Key Laboratory of Sustainable Utilization Research on Rubber Tree / National and Local Joint Engineering Research Center of Breeding and Cultivation Technology of Rubber Tree, Jinghong, Yunnan 666100, China
  • 2.School of Biological and Chemical Science, Puer University, Pu’er, Yunnan 665000, China
  • 3.Rubber Research Institute，Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
Published: 2024-05-25 doi: 10.3969/j.issn.1000-2561.2024.05.015
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The adsorption and desorption rates of nine cationic exchange resins on ergothioneine (EGT) were investigated to screen out the ion exchange resin suitable for the separation and purification of EGT from natural rubber serum (NRS) and determine the optimum process parameters. The results showed that the SA-2 cation exchange resin was the most suitable for the separation and purification of EGT from NRS, and the optimum parameters were as follows: pH of sample loading 3.0-4.0, sample loading flow rate 2 BV/h, and eluted with 0.5% ammonium hydroxide at the flow rate of 1 BV/h. Furthermore, a higher sample loading concentration was more conducive to the adsorption capacity of resin. Under the conditions, the EGT elution rate could reach 97.83%. This research indicated that the SA-2 cation exchange resin is an ideal medium for the separation and purification of EGT from NRS, which would lay a theoretical foundation for the industrial production of EGT.

natural rubber serum (NRS)  /  ergothioneine  /  ion exchange resin
Shikuan JIANG, Juan LI, Guimei ZHANG, Rong XU, Li DING. Separation and Purification of Ergothioneine from Natural Rubber Serum with Ion Exchange Resin[J]. Chinese Journal of Tropical Crops, 2024 , 45 (5) : 1000 -1006 . DOI: 10.3969/j.issn.1000-2561.2024.05.015
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.05.015
  • Receive Date：2023-05-05
  • Online Date：2026-06-23
  • Published：2024-05-25
Article Data
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History
  • Received：2023-05-05
  • Revised：2023-06-25
Funding
Affiliations
    1.Yunnan Institute of Tropical Crops / Yunnan Key Laboratory of Sustainable Utilization Research on Rubber Tree / National and Local Joint Engineering Research Center of Breeding and Cultivation Technology of Rubber Tree, Jinghong, Yunnan 666100, China
    2.School of Biological and Chemical Science, Puer University, Pu’er, Yunnan 665000, China
    3.Rubber Research Institute，Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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