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Anthracnose is a common disease of coffee, which causes serious loss to the yield and quality of coffee fruit. The research on pathogenicity and biological characteristics of the pathogenic bacteria causing coffee anthracnose and screening of effective antagonists would provide reference for the biological control of coffee anthracnose. Four coffee anthracnose pathogens Wyq1 (Colletotrichum siamense), Yyq1 (C. fructicola), Xbd1 (C. gloeosporioides) and Hb1 (C. theobromicola) were isolated and tested for pathogenicity by spore suspension, and the most virulent strains were selected for further experiments. The biological characteristics of the pathogenic bacteria were studied by the plate culture method. Screening of antagonistic bacteria was done by the plate confrontation method. Xbd1 strain could infect healthy coffee leaves, and its virulence was the strongest among the four strains. The most suitable medium for the vegetative growth was PDA. The optimal nitrogen source was tryptone. The most suitable carbon source was glucose. The optimum temperature was 28 ℃. The optimal pH was 7. The difference of vegetative growth was not significant under different light conditions. Bacillus subtilis MC4-2, B. tequilensis D5-8, B. velezensis MC2-1 and B. flexus ZLSY3 and B. amyloliticus GJ7 showed good antagonistic effect in indoor screening. B. subtilis MC4-2 had the best antagonistic effect, with an inhibitory rate of 57.8%. The study would lay the foundation for the control of anthrax and the development of biocontrol agents.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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