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Identification and Genetic Diversity Analysis of Hybrid Offsprings of Curcuma alismatifolia Revealed by SSR Markers
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Suhua JIANG1, Yuxin WU1, Jinrong LUO1, Yuejuan YE1, Jinshui LIN2, Huiwen YU1, *, Luanmei LU1, *
Chinese Journal of Tropical Crops | 2024, 45(5) : 928 - 935
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Chinese Journal of Tropical Crops | 2024, 45(5): 928-935
Germplasm Resources, Genetics & Breeding
Identification and Genetic Diversity Analysis of Hybrid Offsprings of Curcuma alismatifolia Revealed by SSR Markers
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Suhua JIANG1, Yuxin WU1, Jinrong LUO1, Yuejuan YE1, Jinshui LIN2, Huiwen YU1, *, Luanmei LU1, *
Affiliations
  • 1.School of Biological Science and Biotechnology, Minnan Normal University / Fujian Provincial University Key Laboratory of Fujian-Taiwan Plants, Zhangzhou, Fujian 363000, China
  • 2.Zhangzhou Jinluan Horticulture Co., Ltd., Zhangzhou, Fujian 363000, China
Published: 2024-05-25 doi: 10.3969/j.issn.1000-2561.2024.05.007
Outline
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Curcuma alsimatifolia is a perennial bulbous herb of the genus Curcuma in the Gingeraceae.family The inflorescence of C. alismatifolia resembles tulips and enjoys the reputation of “tropical tulips”. C. alsimatifolia was introduced into China from Thailand and it is acclaimed by an ever-increasing number of consumers. The varieties of C. alismatifolia are relying on import in China today. The lack of new varieties with proprietary intellectual property rights limits the development of C. alismatifolia industry in China. Three different cross combinations of 327 hybrid offspring were constructed using the Siam TM Sitrone variety of C. alismatifolia as the female parent to breed new varieties of C. alismatifolia with independent intellectual property rights and increase the variety diversity. Eleven pairs of SSR primers were selected to analyze the hybrid F1 generation of the three cross combinations and the parents, and five pairs of specific SSR primers were selected for each cross combination to identify the authenticity of the hybrid F1 generation. The genetic segregation pattern and genetic diversity and genetic relationship of the parental and hybrid F1 generation populations were also analyzed. The results showed that the eleven pairs of SSR markers had high resolution and good amplification polymorphism for the three parental materials, and each parent had its specific SSR markers, which could effectively identify the genetic authenticity of the hybrid offspring. True hybridization rates of 91.8%, 90.8% and 93.4% were identified in 327 F1 individuals The SSR molecular marker primers had high polymorphism and could be directly used for C. alismatifolia genetic diversity analysis, hybrid identification and other related studies, providing efficient and reliable SSR marker selection for future identification of hybrid progeny, while the identified true C. alismatifolia progeny population could provide germplasm for genetic studies of superior traits. The identification of authentic C. alismatifolia progeny could provide germplasm materials for genetic research on superior traits and provide scientific basis for the selection of new varieties.

Curcuma alismatifolia  /  SSR  /  hybrid F1 progenie  /  hybrid identification  /  genetic diversity
Suhua JIANG, Yuxin WU, Jinrong LUO, Yuejuan YE, Jinshui LIN, Huiwen YU, Luanmei LU. Identification and Genetic Diversity Analysis of Hybrid Offsprings of Curcuma alismatifolia Revealed by SSR Markers[J]. Chinese Journal of Tropical Crops, 2024 , 45 (5) : 928 -935 . DOI: 10.3969/j.issn.1000-2561.2024.05.007
Year 2024 volume 45 Issue 5
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.05.007
  • Receive Date:2022-11-30
  • Online Date:2026-06-23
  • Published:2024-05-25
Article Data
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History
  • Received:2022-11-30
  • Revised:2022-12-30
Funding
Affiliations
    1.School of Biological Science and Biotechnology, Minnan Normal University / Fujian Provincial University Key Laboratory of Fujian-Taiwan Plants, Zhangzhou, Fujian 363000, China
    2.Zhangzhou Jinluan Horticulture Co., Ltd., Zhangzhou, Fujian 363000, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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