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Tobacco is an important economic crop, and its leaves have great economic value. In recent years, with the increase of tobacco planting areas, the varieties of leaf diseases are on the rise. Two strains of tobacco leaf spot disease pathogenic bacteria were isolated from the tobacco leaf disease samples in Hezhou, Guangxi. Through polygenic phylogenetic analysis, they were identified as Cumuliphoma indica, which is the first report on the tobacco leaf spot disease caused by C. indica in China. To clarify the biological characteristics of the pathogen and screen the control agents, this study used the mycelial growth rate method to study the lethal temperature of the mycelia of the strain, and its growth in different media, temperatures, pH, carbon sources, nitrogen sources, light cycles and to determine the sensitivity of the pathogen to nine chemical agents. The biological characteristics showed that the temperature range of C. indica mycelium growth was wide, and the optimum temperature was 28 ℃. Weak acid environment was more favorable for mycelium growth, and the optimal pH was 5. It grew fastest on CMA and CDA. The optimum nitrogen source was peptone. The optimum carbon sources was soluble starch. The lethal temperature of mycelium was 49 ℃, and the water bath was 10 min. Light had a significant effect on the growth of the mycelia, and 24 h full light was the most favorable. The results of laboratory screening showed that the nine tested fungicides had different inhibition on the growth of C. indica mycelia, among which boscalid 50% WG had the best inhibitory effect, EC50 was 0.025 mg/L. The second was fludioxonil 50% FS (0.084 mg/L), iprodione 500 g/L SC (0.217 mg/L), prochloraz 450 g/L EW (1.354 mg/L), mancozeb 80% WP (6.565 mg/L), difenoxazole 10% ME (9.576 mg/L), pyraclostrobin 25% SC (34.237 mg/L) and myclobutanil 40% SC (38.804 mg/L). The inhibitory effect of tebuconazole 430 g/L SC was the weakest, EC50 was 42.574 mg/L. The study revealed the biological characteristics of C. indica for the first time, and recommended 50% acetanilide WG as a candidate fungicide for the prevention and control. The results could provide a reference for the effective prevention and control of tobacco leaf spot disease caused by C. indica.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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