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Gene Silencing of Papaya ringspot virus Replicase Gene (PRSV-Nib) to Obtain Virus Resistant Papaya
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Qinghua WU1, 2, Ruizong JIA2, Jingyuan GUO2, Muzhi YANG2, Yujuan HU2, Zhigang HAO2, Hui ZHAO2, **, Anping GUO2, **
Chinese Journal of Tropical Crops | 2024, 45(4) : 837 - 846
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Chinese Journal of Tropical Crops | 2024, 45(4): 837-846
• Plant Protection & Bio-safety •
Gene Silencing of Papaya ringspot virus Replicase Gene (PRSV-Nib) to Obtain Virus Resistant Papaya
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Qinghua WU1, 2, Ruizong JIA2, Jingyuan GUO2, Muzhi YANG2, Yujuan HU2, Zhigang HAO2, Hui ZHAO2, **, Anping GUO2, **
Affiliations
  • 1.College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
  • 2.Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions / Sanya Research Institutey, Chinese Academy of Tropical Agricultural Sciences / Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572024, China
Published: 2024-04-25 doi: 10.3969/j.issn.1000-2561.2024.04.019
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Papaya is an economically important tropical fruit. Papaya ringspot virus (PRSV) is an important virus disease of papaya, often causing significant yield losses and quality deterioration. Since the introduction of the first transgenic papaya in 1998, PDR-based breeding strategies for disease resistance have been successfully applied. The contradiction between disease resistance based on sequence homology and increased virus genetic diversity became a new challenge for papaya breeding. In this study, we propose to use RNAi strategies aim at nuclear inclusion b gene (Nib) to obtain broad-spectrum resistance to PRSV papaya. With optimized embryo callus generation-Agrobatium meidated transformation-shoot regeneration, 52 shoots were obtained after resistance screening and a total of 24 transgenic positive shoots were obtained by specific PCR screening for the T0 generation. In the T0 generation field natural disease test, the transgenic papaya strains were significantly more resistant to disease than the non-transgenic control, with NibB5-2 having the best resistance in the field. Molecular charaterization of the insertion site of Nib5-2 was located at position 1976766 on chromosome 2 supercontig_30 by hiTAIL-PCR method. Green house inoculation test of NibB5-2 T1 generation, there was no virus accumulation and no disease symptoms, confirmed the resistance. Our resulst reveal that RNAi silcencing the Nib gene enhance the papaya virus resistance, meanwhile enriched the candidated genes for virus resistance breeding.

Carica papaya L.  /  Papaya ringspot virus  /  Nib gene  /  RNA-mediated virus resistance
Qinghua WU, Ruizong JIA, Jingyuan GUO, Muzhi YANG, Yujuan HU, Zhigang HAO, Hui ZHAO, Anping GUO. Gene Silencing of Papaya ringspot virus Replicase Gene (PRSV-Nib) to Obtain Virus Resistant Papaya[J]. Chinese Journal of Tropical Crops, 2024 , 45 (4) : 837 -846 . DOI: 10.3969/j.issn.1000-2561.2024.04.019
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Year 2024 volume 45 Issue 4
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.04.019
  • Receive Date：2022-12-16
  • Online Date：2026-06-23
  • Published：2024-04-25
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History
  • Received：2022-12-16
  • Revised：2023-02-15
Funding
Affiliations
    1.College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2.Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions / Sanya Research Institutey, Chinese Academy of Tropical Agricultural Sciences / Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572024, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2024.04.019
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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