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A time-saving, efficient and environmentally friendly method for the preparation of soluble dietary fiber (SDF) from coffee peel with ultrasound-assisted low eutectic solvents (UAE-DESs) was established for the first time, and its process parameters were optimized by single-factor test and response surface test to further explore a new green method for the preparation of soluble dietary fiber from coffee peel. Eight choline chloride-based deep eutectic solvents were prepared as extraction solvents by the heating method and compared with the conventional water extraction method. The results showed that the deep eutectic solvent system with a 1∶2 molar ratio of choline chloride to urea prepared the highest yield of soluble dietary fiber from coffee peel at 10.10%±0.11%, which was significantly higher than that of the conventional water extraction method at 6.50%±0.22%. The effects of DES composition (molar ratio, water content, liquid-to-solid ratio) and ultrasonic treatment (ultrasonic power, ultrasonic time) on the yield of soluble dietary fiber from coffee peel were investigated by single-factor experiments. The response surface methodology (RSM) combined with Box-Behnken design (BBD) was used to optimize the soluble dietary fiber yield and investigate the effects of deep eutectic solvent water content, liquid-solid ratio, ultrasonic power and ultrasonic time on the yield of soluble dietary fiber. Based on the optimization results of RSM, the optimal preparation conditions were determined as follows: water content of deep eutectic solvent 39.91%, liquid-solid ratio 32.37 mL/g, ultrasonic power 305.20 W, and ultrasonic time 33.80 min. Under this condition, the maximum soluble dietary fiber yield of coffee peel was 10.74%±0.14%, which was close to the predicted soluble dietary fiber value of 10.80%. Therefore, as an efficient and non-polluting green solvent, deep eutectic solvent can be used for the preparation of soluble dietary fiber from coffee peel. The results of this study would provide new ideas and theoretical references for the preparation of soluble dietary fiber from coffee peel, and have potential applications in the value-added utilization of coffee by-products, the production of new high value-added products and the development of natural functional foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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