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The study was aimed to screen out the excellent tobacco germplasm resources which could be used in the field of scientific research, production and breeding. Conventional chemical composition and major alkaloids were tested, compared and clustered for 141 flue-cured tobacco, 18 burly tobacco and 6 cigar germplasm resources from China and abroad. Total sugar and reducing sugar contents were high in flue-cured tobacco germplasms, while total nitrogen, nicotine and potassium oxide were high in burley tobacco germplasms. Total alkaloid and nicotine content were the highest in flue-cured tobacco germplasm Zilaihuang 2243, and the content of cigar germplasm Dutch (ohio) was the lowest. The nicotine and nicotine conversion rates were the highest in the tobacco germplasm Pingbanliu, and those for tobacco germplasm Erxingzi and Burley11B was the lowest. The 165 accessions were divided into 4 groups for clustering analysis. Flue-cured tobacco germplasms were mainly concentrated in Group 1 and 4, characterized by high total and reducing sugar content and low nicotinic conversion rate. Group 2 was low in total and reducing sugars, and high in total nitrogen, total alkaloids and potassium oxide. Group 3 had low nicotinoids and total alkaloids, high nicotinic reduction, and high nicotinic conversion rate. The chemical composition and alkaloids of different germplasm resources have significant difference. The selected germplasms with different gradients could provide useful material for further study of nicotine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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