PDF
Full
Outline
Dioscorea esculenta (Lour.) brukill is a traditional miscellaneous grain in Hainan, and it is rich in polysaccharides. This study investigated the extraction process and anti-oxidant activity of the polysaccharides from D. esculenta by using the D. esculenta tuber powder as the raw material. The hot water extraction was used to optimize the extraction process of polysaccharides from D. esculenta by single factor experiments and three-factor and three-level L9(33) orthogonal tests. The crude polysaccharides from D. esculenta were purified by the Sveag method, dialysis, DEAE-52 cellulose column and SephadexG-100 gel column chromatography. The anti-oxidant activity of polysaccharide from D. esculenta was detected by DPPH method, hydroxyl radical method, superoxide anion method and reduction method. The optimal extraction method of the hot water extraction included temperature 70 ℃, solid-liquid ratio 1∶15, and extraction time 3 h. The polysaccharides extraction rate of D. esculenta was 1.94%. After multipleseparation and purification, neutral polysaccharides with uniform purity were obtained. The expriments of anti-oxidant activity of polysaccharides from D. esculenta showed that the DPPH radical scavenging capacity of polysaccharide from D. esculenta was weak. The polysaccharide from D. esculenta and scavenging rate of 1-5 mg/mL was maintained at about 10%, and with the increase of polysaccharide concentration, the scavenging capacity gradually decreased. The hydroxyl radical activity increased with the increase of polysaccharide concentration, and the scavenging rate reached 34.74% at 5 mg/mL. The superoxide anion free radical activity decreased gradually with the increase of polysaccharide concentration, and the scavenging rate reached 67.35% at 1 mg/mL. The reduction capacity of polysaccharides was weak, and the reduction capacity of 1-5 mg/mL remained 0.17. In this study, the extraction process of polysaccharides from D. esculenta was optimized, and it had certain antioxidant activity. It would lay the foundation for the functional product development and the mechanism research of drug function of D. esculenta.
PDF
38
6
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
