Military Medical Research

Military Medical Research ›› 2023, Vol. 10 ›› Issue (4) : 529 - 553. DOI: 10.1186/s40779-022-00434-8

REVIEW

Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications

Min Su ¹^,^† ,
Tao Pan ²^,^† ,
Qiu-Zhen Chen ¹^,^† ,
Wei-Wei Zhou ³^,^† ,
Yi Gong ¹^,⁴ ,
Gang Xu ² ,
Huan-Yu Yan ¹ ,
Si Li ² ,
Qiao-Zhen Shi ¹ ,
Ya Zhang ² ,
Xiao He ⁵ ,
Chun-Jie Jiang ⁶ ,
Shi-Cai Fan ⁷ ,
Xia Li ³^,^* ,
Murray J. Cairns ⁸^,⁹^,^* ,
Xi Wang ¹^,^* ,
Yong-Sheng Li ²^,^*

Author information +

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China

²College of Biomedical Information and Engineering, the First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China

³College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, China

⁴Department of Immunology, Nanjing Medical University, Nanjing 211166, China

⁵Department of Laboratory Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing 401174, China

⁶Baylor College of Medicine, Houston, TX 77030, USA

⁷Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Shenzhen 518110, Guangdong, China

⁸School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, the University of Newcastle, University Drive, Callaghan, NSW 2308, Australia

⁹Precision Medicine Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia

^*lixia@hrbmu.edu.cn;

murray.cairns@newcastle.edu.au;

xiwang@njmu.edu.cn;

liyongsheng@hainmc.edu.cn

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Issue Date
2025-11-27