Home Journals Articles Updates About
CN
image
收藏切换
Surveillance of arthropod-borne viruses in Benin, West Africa 2020–2021: detection of dengue virus 3 in Aedes aegypti (Diptera: Culicidae)
收藏切换
PDF
Carine Tchibozo1, Gildas Hounkanrin1, Anges Yadouleton1, 2, 3, Alexandra Bialonski4, Eric Agboli4, 5, Renke Lühken4, Jonas Schmidt-Chanasit4, 6, Hanna Jöst4, *
Military Medical Research | 2023, 10(5) : 730 - 732
Less
收藏切换
Military Medical Research | 2023, 10(5): 730-732
• LETTER TO THE EDITOR •
Surveillance of arthropod-borne viruses in Benin, West Africa 2020–2021: detection of dengue virus 3 in Aedes aegypti (Diptera: Culicidae)
Full
Carine Tchibozo1, Gildas Hounkanrin1, Anges Yadouleton1, 2, 3, Alexandra Bialonski4, Eric Agboli4, 5, Renke Lühken4, Jonas Schmidt-Chanasit4, 6, Hanna Jöst4, *
Affiliations
  • 1Laboratory of Viral Haemorrhagic Fevers and Arboviruses of Benin, 01BP 918, Cotonou, Benin
  • 2Centre de Recherche Entomologique de Cotonou, 01BP 918, Cotonou, Benin
  • 3Ecole Normale Supérieure de Natitingou, National University of Science, Technology, Engineering and Mathematics, Abomey, Benin
  • 4Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, 20359 Hamburg, Germany
  • 5University of Health and Allied Sciences, PMB 31, Ho, Ghana
  • 6Faculty of Mathematics, Informatics and Natural Sciences, Universität Hamburg, 22609 Hamburg, Germany
Published: 2023-10-10 doi: 10.1186/s40779-022-00425-9
Outline
收藏切换
Dengue virus  /  Arbovirus  /  Mosquitoes  /  Aedes aegypti  /  Benin
Carine Tchibozo, Gildas Hounkanrin, Anges Yadouleton, Alexandra Bialonski, Eric Agboli, Renke Lühken, Jonas Schmidt-Chanasit, Hanna Jöst. Surveillance of arthropod-borne viruses in Benin, West Africa 2020–2021: detection of dengue virus 3 in Aedes aegypti (Diptera: Culicidae)[J]. Military Medical Research, 2023 , 10 (5) : 730 -732 . DOI: 10.1186/s40779-022-00425-9
Full Text
Less
Dear Editor,
Dengue virus (DENV, family Flaviviridae, genus Flavivirus) serotypes 1 to 4 (DENV-1, -2, -3, and -4) are responsible for more than 100 million infections per year worldwide. Symptoms of DENV infection can be diverse, reaching from an acute febrile illness to the more severe, sometimes fatal dengue haemorrhagic fever/dengue shock syndrome. After the primary infection, lifelong immunity against a specific serotype is built. However, secondary infections by heterologous serotypes increase the risk of severe disease. First epidemics in Africa date back to the 19th century, and since the 1960s laboratory-confirmed cases and outbreaks have been reported in many countries in sub-Saharan Africa[1]. All four serotypes circulate in Africa with DENV-1 and DENV-2 being described most frequently. Due to the lack of specific diagnostics, DENV infections are often misdiagnosed with diseases that share a similar clinical presentation, e.g., malaria, chikungunya, Zika, yellow fever, or typhoid fever. A valuable, non-invasive tool to detect the circulation of arthropod-borne viruses (arboviruses) is entomological surveillance. Monitoring arboviruses in field-collected mosquitoes can serve as a tool to guide the appropriate control measures prior to arbovirus epidemics. In addition, it allows the investigation of arbovirus evolution or adaptation, as well as the identification of new lineages, and provides data about vectors that are involved in the local arbovirus transmission.
In Africa, data about arbovirus distribution and prevalence mostly come from seroprevalence surveys or single case studies, i.e., travellers returning to countries outside of Africa. Reported cases are suggested to significantly underestimate the underlying arboviral disease burden. In Benin, a country with a tropical wet-dry climate, the serological evidence of DENV infections in travellers returning from Benin have been described[2]. To gain data about the occurrence of arboviruses, a longitudinal arbovirus surveillance study was conducted.
Mosquitoes were collected from June 2020 until October 2021 at five sites in two bioclimatic regions in Benin (Additional file 1: Fig. S1). Specimens were morphologically identified on chilled tables and samples were tested with pan-Flavivirus, pan-Alphavirus and pan-Orthobunyavirus reverse transcription-polymerase chain reaction (RT-PCR) with primers shown in the Additional file 1: Table S1. The amplicons were sent for sequencing (LGC Genomics, Berlin, Germany) and sequences were analysed with Geneious v9.1.7 (Biomatters, Auckland, New Zealand).
A total of 3749 female mosquitoes, including two genera and four species, were captured during the sampling period (Additional file 1: Table S2). The dominant species was Aedes aegypti with 2970 specimens, followed by Culex quinquefasciatus with 753 specimens. DENV-3 specific RNA was detected in one sample (pool of 25 Aedes aegypti) collected with human landing catch (HLC) on 7th July 2021 in Porto Novo. Phylogenetic analysis revealed that the DENV-3 strain from Benin is grouped into the genotype III clade. The sequence clusters in a distinct clade within a monophyletic Africa clade (Fig. 1). Sequence analysis showed a close relationship to DENV-3 strains from humans in Burkina Faso and Ethiopia with a paired identity at the nucleotide level of 98.8% and 98.5%, respectively.
We report the first detection of DENV in mosquitoes in Benin, giving another indication that this virus is endemically occurring in this region. Phylogenetic analysis showed a close relationship to DENV strains from Burkina Faso and Ethiopia. Burkina Faso experienced a greater DENV outbreak in 2016 and 2017. The detection of DENV-3 genotype III strain in Benin and surrounding countries supports the suggestion that there is an ongoing circulation of different DENV strains in the West African countries. Specifically, DENV-3, has become increasingly common in West and Central Africa in the last decade and has been responsible for several outbreaks in Senegal (DENV-3 accounting for 65% of all gained sequences)[3], Burkina Faso[4] and Gabon[5]. Until now, Benin did not report any outbreak of DENV, but it must be assumed that human infections occur regularly. Due to the restricted availability of diagnostic capacity, most investigations are performed only during clearly noticeable outbreak periods. Our study highlights that there is a need to implement further investigations and surveillance strategies to prevent and control future outbreaks of mosquito-borne viruses in Western Africa.
Abbreviations
Less
DENV: Dengue virus; HLC: Human landing catches; RNA: Ribonucleic acid; RT-PCR: Reverse transcription polymerase chain reaction.
Supplementary information
Less
The online version contains supplementary material available at https://doi.org/10.1186/s40779-022-00425-9.
Additional file 1: Fig. S1 Map of Benin with trapping sites and number of collected mosquitoes. Table S1 List of the primer sequences used for reverse transcription polymerase chain reaction. Table S2 Total number of collected female mosquitoes.
Acknowledgements
Less
We thank Daniel Cadar for the valuable assistance in the preparation of the manuscript.
Authors’ contributions
Less
The study was conceived and designed by AY, HJ, JSC and RL. Data were collected by CT and GH. The data were analysed by CT, GH, AB and EA. The manuscript was drafted by AY, HJ and EA. The manuscript was critically revised by JSC and RL. All authors approved the final version of the manuscript.
Availability of data and materials
Less
Not applicable.
Declarations
Less
Ethics approval and consent to participate
Ethical approval for this study was granted by the Ethical Committee of the Ministry of Health in Benin (045/MS/DC/SGM/DNSP/CJ/SA/027SGG2020). Verbal consent was asked to the head of each household for mosquito collection and consent of collectors was obtained prior to HLC. They received anti-malaria prophylaxis and yellow fever immunization. They were organized in teams of two for each collection point and they rotated between locations within houses every two hours. In case of refusal, permission was sought from the next household.
Consent for publication
Less
Not applicable.
Competing interests
Less
The authors declare that they have no competing interests.
Author details
Less
1Laboratory of Viral Haemorrhagic Fevers and Arboviruses of Benin, 01BP 918, Cotonou, Benin. 2Centre de Recherche Entomologique de Cotonou, 01BP 918, Cotonou, Benin. 3Ecole Normale Supérieure de Natitingou, National University of Science, Technology, Engineering and Mathematics, Abomey, Benin. 4Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, 20359 Hamburg, Germany. 5University of Health and Allied Sciences, PMB 31, Ho, Ghana. 6Faculty of Mathematics, Informatics and Natural Sciences, Universität Hamburg, 22609 Hamburg, Germany.
Funding
Less
  • German Research Foundation(JO 1276/5-1)
  • Federal Ministry of Education and Research of Germany (BMBF) under the project NEED(01Kl2022)
References
Less
1.
Agboli E, Zahouli JBZ, Badolo A, Jöst H. Mosquito-associated viruses and their related mosquitoes in West Africa. Viruses. 2021; 13(5): 891.
2.
Fourié T, Luciani L, Amrane S, Zandotti C, Leparc-Goffart I, Ninove L, et al. Dengue virus type 1 infection in traveler returning from Benin to France, 2019. Emerg Infect Dis. 2020; 26(8): 1946-9.
3.
Gaye A, Ndiaye T, Sy M, Deme AB, Thiaw AB, Sene A, et al. Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018. Sci Rep. 2021; 11(1): 10321.
4.
Letizia AG, Pratt CB, Wiley MR, Fox AT, Mosore M, Agbodzi B, et al. Retrospective genomic characterization of a 2017 dengue virus outbreak. Burkina Faso Emerg Infect Dis. 2022; 28(6): 1198-210.
5.
Abe H, Ushijima Y, Loembe MM, Bikangui R, Nguema-Ondo G, Mpingabo PI, et al. Re-emergence of dengue virus serotype 3 infections in Gabon in 2016–2017, and evidence for the risk of repeated dengue virus infections. Int J Infect Dis. 2020; 91: 129-36.
Appendix
Less
Year 2023 volume 10 Issue 5
PDF
46
25
Cite this Article
BibTeX
Article Info
doi: 10.1186/s40779-022-00425-9
  • Online Date：2025-11-27
  • Published：2023-10-10
Article Data
Affiliations
History
Funding
German Research Foundation(JO 1276/5-1)
Federal Ministry of Education and Research of Germany (BMBF) under the project NEED(01Kl2022)
Affiliations
    1Laboratory of Viral Haemorrhagic Fevers and Arboviruses of Benin, 01BP 918, Cotonou, Benin
    2Centre de Recherche Entomologique de Cotonou, 01BP 918, Cotonou, Benin
    3Ecole Normale Supérieure de Natitingou, National University of Science, Technology, Engineering and Mathematics, Abomey, Benin
    4Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, 20359 Hamburg, Germany
    5University of Health and Allied Sciences, PMB 31, Ho, Ghana
    6Faculty of Mathematics, Informatics and Natural Sciences, Universität Hamburg, 22609 Hamburg, Germany

Corresponding:

References
Share
https://castjournals.cast.org.cn/joweb/mmr/EN/10.1186/s40779-022-00425-9
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House