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Outline
NAC transcription factors are pivotal in regulating plant growth, development, and stress responses. This study aims to investigate the role of the TaNAC2 gene in wheat's adaptation to low-temperature stress and its growth development. To achieve this, we developed TaNAC2 gene overexpression and gene-edited lines using Fielder as the transformation recipient. Subsequently, we evaluated cold stress tolerance, growth development, and agronomic traits in both controlled incubator and greenhouse. The results showed that after 10 hours of low-temperature stress treatment at -5 ℃, compared to Fielder, the relative expression level of TaNAC2 genes in the T3 overexpression lines OE8, OE11, and OE14 was significantly increased (P<0.01), while relative conductivity was significantly reduced (P<0.05); seedling survival rates increased and frost damage indices decreased, but neither showed significant differences. In the T4-generation gene-edited line GE45 (aabbdd), the relative expression level of the TaNAC2 gene was significantly reduced (P<0.05); seedling survival rate decreased, and relative conductivity and frost injury index increased, but none of these showed significant differences; in GE91 (aaBBDD), the relative expression level of the TaNAC2 gene, relative conductivity, seedling survival rate, and frost injury index were all similar to Fielder. Under greenhouse pot conditions, compared with Fielder, except that the days from sowing to heading of OE11 were significantly reduced (P<0.05), there were no significant differences in plant height, tiller number, and spikelet number of OE8, OE14, GE45, and GE91. In summary, the TaNAC2 gene positively regulates wheat's tolerance to low-temperature stress, but has no obvious effect on agronomic traits.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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