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Outline
To broaden the genetic resources for salt-tolerant wheat, 96 wheat-Thinopyrum ponticum derivative lines were treated with 150 mmol·L-1 NaCl at the seedling stage. Fourteen morphological traits were measured, and a comprehensive evaluation of salt tolerance was carried out using principal component analysis (PCA), the membership function method, and cluster analysis. The results showed that salt stress significantly inhibited both aboveground and underground growth of the seedlings. Most traits, such as shoot fresh weight and root fresh weight, decreased markedly compared to the control, with high coefficients of variation (>25%). PCA extracted five principal components, which together explained 83.85% of the total variance. Among them, a biomass factor (including shoot fresh weight, root fresh weight, total root length, root surface area, shoot dry weight, and root dry weight) had the highest contribution rate (33.15%), which can be used as a key indicator for evaluating salt tolerance at the seedling stage. Cluster analysis classified the 96 lines into five salttolerance grades, including seven highly salttolerant lines (7.1%) and 17 moderately salt-tolerant lines (17.3%). Further measurements of Na+ and K+ contents confirmed that salttolerant lines maintained a lower Na+/K+ ratio under salt stress, showing a stronger ability to regulate ion homeostasis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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