To gain a compound microbial seed coating formulation with synergistic efficacy against wheat crown rot, Bacillus paralicheniformis ZYGT1811 and Bacillus atrophaeus ZY1825 were selected as the primary active components of the seed coating agent. Single-factor experiments, orthogonal tests, and the filter paper disc diffusion method were employed to screen for the optimal dispersant, film-forming agent, and the optimal mixing ratio of the seed coating agent. The results indicated that no significant mutual antagonism was observed between ZYGT1811 and ZY1825, providing a basis for their compounding. When the ratio of ZYGT1811 to ZY1825 was 2∶1, the synergistic inhibition rate against Fusarium pseudograminearum MC-7 reached 64.17%, which was significantly higher than the inhibition rates of the single-strain treatments(58.12% and 60.20%, respectively). Furthermore, the optimal auxiliary system formulation for the seed coating agent was determined as follows: 4% sodium lignosulfonate, 5% hydroxypropyl methylcellulose, 1.5% ethylene glycol, 0.1% xanthan gum, and 2% scarlet dye. Physicochemical property tests demonstrated that the compound microbial seed coating complied with GB/T 17768-1999 and relevant industrial standards. Its shelf life was up to 90 days, and the pot control efficacy against wheat crown rot was 60.56%.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |