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An investigation on wheat disease in Jiangsu Province was carried out and the samples showed that the tip of the flag leaf expanded to the leaf base and became yellow, and the yellowing accounted for about 1/3 to 1/2 of the whole leaf, which was very similar with the symptoms induced by barley yellow dwarf viruses (BYDV). In order to detect and identify the possible virus associated with the disease, the samples were collected from eight locations in Jiangsu province. A total of 261 samples were detected with various wheat viruses using RT-PCR and 182 samples were found positive when a pair of degenerate primers for BYDV was used. The result of sequence analysis revealed that the virus shared the highest sequence identities with BYDV-PAV. The positive samples were further detected using specific primers for BYDV-PAV coat protein gene (CP) and the results showed that all samples were positive. Further phylogenetic analysis based on the sequence of BYDV-PAV CP gene revealed that the virus detected shared the highest sequence identities with the isolates from Korea. This is the first report of barley yellow dwarf virus-PAV in Wheat grown in Jiangsu province, China.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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