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Outline
The 1N chromosome of Aegilops uniaristata contains superior genes essential for wheat breeding. To rapidly and accurately identify the 1N chromosome of Aegilops uniaristata from a wheat background, this study utilized 212 primer pairs to analyze Aegilops uniaristata, a set of wheat-Aegilops uniaristata addition lines, with a wheat material as control. The results showed that after digestion with Taq Ⅰ enzyme, the amplification products of primers TNAC1006, TNAC1017, TNAC1029, and TANC1031 yielded polymorphic fragments of 900, 1200, 1800, and 400 bp in Aegilops uniaristata and the wheat-Aegilops uniaristata 1N addition lines, respectively. These polymorphic fragments were not observed in other tested materials, and thus they can serve as specific molecular markers for detecting 1N chromosome in a wheat background. Using these established molecular markers, the wheat-Aegilops uniaristata 1N addition lines and the F2 population of Chinese Spring hybrids were screened. Simultaneously, dual-color FISH with Oligo-pSc119.2-1 and Oligo-pTa535-1 as probes was used to identify the corresponding individual plants. It was found that plants N91 and N350 contain heterozygous 1NS·1NS isochromosomes; N46 contains a heterozygous 1N+1NL·1NL chromosome; and N319 contains a heterozygous 1BS·1NS translocation chromosome. Further molecular marker and FISH analysis of the 207 self-pollinated progeny from these four plants yielded only one homozygous wheat-Aegilops uniaristata 1NS·1NS translocation line. To investigate the reason for the extremely low proportion of homozygous translocation lines obtained, a test cross was conducted using the 1BS·1NS+1B heterozygous line and Jimai 38 as materials. The results confirmed that 1BS·1NS is transmitted only through female gametes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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