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In the spring of 2022, wheat plants showed suspected viral disease symptoms of chlorosis, yellowing and dwarfing in Nanjing and Huai'an in Jiangsu Province. To verify the virus species, the pathogen molecular identification and phylogenetic analysis were carried out. The results showed a high incidence (53.6% and 86.7%) of barley yellow dwarf virus-PAV (BYDV-PAV) in the samples using the universal primer Lu-1/Lu-4 for Luteoviruses. When the universal Leu-F/Leu-R primer was used, a target fragment (624 bp) was amplified in partial samples. Sequencing analysis suggested that the four amplicons from Nanjing samples shared the highest homology (97.78%) with wheat yellow dwarf virus (WYDV), and the four amplicons from Huai'an samples had the highest homology (97.60%) with panicum distortion mosaic virus (PDMV). In order to further identify virus species, the specific primers against coat proteins (CP) of the two viruses were designed for RT-PCR detection, and the target fragments can be amplified from positive samples. The cloning and sequencing results revealed that CP gene of Nanjing isolate was 612 bp in full-length, with 98.69% homologous to WYDV Henan isolate (OK216142), and CP gene of Huai'an isolate was 615 bp in full-length, sharing 97.89% and 97.72% nucleotide identity to PDMV Hubei isolate (OM514390) and Korean isolate (LC424839), respectively. Phylogenetic analysis suggested that the two viruses were clustered into the branch of Poleroviruses. These results indicate that the two novel viruses detected in wheat from Nanjing and Huai'an are the isolates of WYDV and PDMV, respectively, which is the first report of the two viruses infected in crops in Jiangsu Province.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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