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Outline
Ae. umbellulata (Aegilops umbellulata, 2n=2x=14, UU) is a tertiary gene resource for wheat improvement. In order to accurately identify the chromosomes of Ae. umbellulata U genome or the chromosome segments transferred into wheat, based on the next-generation sequencing information of Ae. umbellulata U genome, 13 oligonucleotide probes were designed for Oligo-FISH analysis according to the 17 possible specific satellite repeats identified. The Oligo-FISH results showed that 10 of the probes exhibited obvious hybridization signals on the chromosomes of Ae. umbellulata. The probe specificity analysis revealed that the five probes generated hybridization signals on the chromosomes of Ae. umbellulata, but there was no obvious hybridization signal on the chromosomes in wheat, which can be used as the specific probes to identify the chromosomes of U genome in wheat background. Two probes Oligo-pAu153 and Oligo-pAu263, generating rich and strong signals on Ae. umbellulata chromosome, and one probe Oligo-pAu133 specific for Ae. umbellulata chromosome combining with Oligo-pSc119.2 were used as a probe multiplex ONPS#AU1. The Oligo-FISH karyotype of Ae. umbellulata was constructed, which can accurately identify each chromosome of the U genome. It will accelerate the step for mining, transferring and utilizing the excellent genes of Ae. umbellulata.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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